High performance liquid chromatography column efficiency enhancement by zero dead volume recycling and practical approach using park and recycle arrangement
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
29706399
DOI
10.1016/j.chroma.2018.03.058
PII: S0021-9673(18)30385-6
Knihovny.cz E-resources
- Keywords
- Circulation chromatography, Closed loop recycling, Peak recycling, Recycle system, Sample recycler,
- MeSH
- Benzene Derivatives chemistry isolation & purification MeSH
- Polycyclic Compounds chemistry isolation & purification MeSH
- Countercurrent Distribution MeSH
- Recycling * MeSH
- Stereoisomerism MeSH
- Chromatography, High Pressure Liquid instrumentation methods MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Benzene Derivatives MeSH
- helicenes MeSH Browser
- Polycyclic Compounds MeSH
A new instrumental approach to recycling HPLC is described. The concept is based on fast reintroduction of incremental peak sections back onto the separation column. The re-circulation is performed within a closed loop containing only the column and two synchronized switching valves. By having HPLC pump out of the cycle, the method minimizes peak broadening due to dead volume. As a result the efficiency is dramatically increased allowing for the most demanding analytical applications. In addition, a parking loop is employed for temporary storage of analytes from the middle section of the separated mixture prior to their recycling.
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