Peripheral venous vs. capillary microfilariaemia in a dog co-infected with Dirofilaria repens and D. immitis: A comparative approach using triatomine bugs for blood collection
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29907194
DOI
10.1016/j.vetpar.2018.05.017
PII: S0304-4017(18)30210-3
Knihovny.cz E-zdroje
- Klíčová slova
- Co-infection, Dirofilaria, Periodicity, Triatomine bug,
- MeSH
- cirkadiánní rytmus MeSH
- Dirofilaria immitis izolace a purifikace fyziologie MeSH
- Dirofilaria repens izolace a purifikace fyziologie MeSH
- dirofilarióza diagnóza parazitologie MeSH
- kapiláry parazitologie MeSH
- koinfekce diagnóza parazitologie veterinární MeSH
- mikrofilárie klasifikace izolace a purifikace MeSH
- nemoci psů diagnóza parazitologie MeSH
- odběr vzorku krve metody veterinární MeSH
- parazitemie diagnóza parazitologie veterinární MeSH
- psi MeSH
- studie proveditelnosti MeSH
- Triatominae * MeSH
- vény parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Dirofilaria immitis and D. repens are mosquito-borne nematodes, primarily infecting dogs, but also other species of carnivores and even humans. Given their impact on animal and human health, the transmission of these filarioids has been widely studied. The microfilariaemia has been shown to have a circadian variation for both Dirofilaria species infecting dogs. Due to methodological difficulties, the periodicity was only studied using venous blood samples, while the mosquitoes feed, in fact, on capillary blood. In this context, the present study aimed to test the feasibility of using triatomine bugs for the collection of capillary blood and to comparatively evaluate the level of microfilariaemia and its circadian variation in capillary blood vs. peripheral venous blood in a dog naturally co-infected with D. immitis and D. repens. The results showed a feeding success of 50%, with variations in the blood meal volume that the bugs ingested. The relative values of microfilariaemia (mf/bug) were strongly correlated with the volume of blood recovered: the more blood recovered from each bug, the higher values of microfilariaemia in the evening samples while the opposite results were obtained for the morning samples. The counting of microfilariae revealed a dominance of D. immitis in all the samples, but with significantly higher microfilariaemia in the venous blood. Meanwhile, for D. repens, the situation was opposite, with higher counts in the capillary blood samples. Our study showed that triatomine bugs can be used as a model for the collection and study of microfilariaemia in the capillary blood in mammals.
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