New fluorescently labeled auxins exhibit promising anti-auxin activity
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
29953966
DOI
10.1016/j.nbt.2018.06.003
PII: S1871-6784(17)30572-1
Knihovny.cz E-resources
- Keywords
- Anti-auxin, Arabidopsis, Biological activity, DR5::GUS, Fluorescent labeling, Indole-3-acetic acid (IAA),
- MeSH
- Arabidopsis drug effects genetics growth & development MeSH
- Fluorescent Dyes chemical synthesis chemistry MeSH
- Spectrometry, Fluorescence MeSH
- Plants, Genetically Modified MeSH
- Plant Roots drug effects growth & development MeSH
- Indoleacetic Acids antagonists & inhibitors chemistry pharmacology MeSH
- Molecular Structure MeSH
- Plant Growth Regulators antagonists & inhibitors chemistry pharmacology MeSH
- Spectrophotometry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Indoleacetic Acids MeSH
- Plant Growth Regulators MeSH
The plant hormone auxin is a key player in the regulation of plant growth and development. Despite numerous studies devoted to understanding its role in a wide spectrum of physiological processes, full appreciation of its function is linked to a comprehensive determination of its spatio-temporal distribution, which plays a crucial role in its mode of action. Conjugation of fluorescent tracers to plant hormones enables sensitive and specific visualization of their subcellular and tissue-specific localization and transport in planta, which represents a powerful tool for plant physiology. However, to date, only a few fluorescently labeled auxins have been developed. We report the synthesis of four novel fluorescently labeled derivatives of indole-3-acetic acid (IAA) in the form of a conjugate with a nitrobenzoxadiazole (NBD) fluorophore together with validation of their biological activity. These compounds, unlike other previously reported auxins fluorescently labeled at N1 position (nitrogen of the indole ring), do not possess auxin activity but rather show dose-dependent inhibition of auxin-induced effects, such as primary root growth inhibition, root hair growth and the auxin reporter DR5::GUS expression. Moreover, the study demonstrates the importance of the character of the linker and optimal choice of the labeling site in the preparation of fluorescently labeled auxins as important variables influencing their biological activity and fluorescent properties.
References provided by Crossref.org
On the trail of auxin: Reporters and sensors
Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis
What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo
