A recently described putative foveavirus, grapevine virus T (GVT), was detected in a Slovak grapevine accession (SK704) using high-throughput sequencing, prompting further studies. Full-length genome sequence of isolate GVT-SK704 was determined. Analyses revealed 86.1% nucleotide identity with the Italian GVT isolate, currently the only available nearly complete sequence of GVT in GenBank. A virus-specific RT-PCR assay was developed, which enabled a survey of GVT incidence in grapevine samples from Slovakia and Czech Republic. Unexpectedly, GVT was present in ~ 30% of tested samples. Analysis of complete CP gene sequences of 20 Slovak and Czech GVT isolates detected in the survey revealed relatively high intra-species variability (up to 11.2% nucleotide divergence), suggesting multiple introductions from different sources, possibly over an extended period of time.

Comenius University Science Park Comenius University Ilkovičova 8 841 04 Bratislava Slovakia

Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Mississippi State MS 39762 USA

Department of Plant Pathology Instituto Valenciano de Investigaciones Agrarias Carretera Moncada a Náquera km 4 5 Moncada 46113 Valencia Spain

Institute of Plant Protection DLR Rheinpfalz Breitenweg 71 67435 Neustadt an der Weinstrasse Germany

Institute of Virology Biomedical Research Centre Slovak Academy of Sciences Dúbravská cesta 9 84505 Bratislava Slovakia

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