Ultrastructural, cytochemistry and electron tomography analysis of Caryophyllaeides fennica (Schneider, 1902) (Cestoda: Lytocestidae) reveals novel spermatology characteristics in the Eucestoda
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
2/0104/16
Grant Agency of the Slovak Republic VEGA
AV ČR-16-08
AV ČR
SAV-18-21
AV ČR
SAV-18-21
SAV
CEP Register
(LM2015062 Czech-BioImaging
MEYS CR
PubMed
30022291
DOI
10.1007/s00436-018-6001-9
PII: 10.1007/s00436-018-6001-9
Knihovny.cz E-resources
- Keywords
- Caryophyllaeides fennica, Caryophyllidea, Spermatozoon, Spermiogenesis, Ultrastructure,
- MeSH
- Axoneme ultrastructure MeSH
- Cell Nucleus ultrastructure MeSH
- Cestoda ultrastructure MeSH
- Cestode Infections MeSH
- Flagella ultrastructure MeSH
- Microtubules ultrastructure MeSH
- Spermatogenesis physiology MeSH
- Spermatozoa ultrastructure MeSH
- Electron Microscope Tomography MeSH
- Microscopy, Electron, Transmission MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Spermatozoon formation in Caryophyllaeides fennica (Schneider, 1902) is characterised by the following: (1) apical electron-dense material in the zone of differentiation, (2) typical striated roots situated unconventionally in opposite directions in early stages of spermiogenesis, (3) intercentriolar body composed of three electron-dense and two electron-lucent plates, (4) free flagellum and a flagellar bud that correspond to a greatly reduced flagellum and (5) rotation of free flagellum and a flagellar bud to the median cytoplasmic process at 90°. The development of two flagella of significantly unequal length clearly supports a derived form of spermiogenesis in the Caryophyllidea. New for cestodes is a finding of two additional striated roots situated opposite each other, in conjunction with both the flagellar bud and free flagellum. Mutual position of additional striated roots and typical striated roots is parallel in early stages and perpendicular in advanced stages of spermiogenesis. A complete proximodistal fusion gives rise to a mature spermatozoon consisting of one axoneme, parallel cortical microtubules, a nucleus and a moderately electron-dense cytoplasm with glycogen particles, detected by a technique of Thiéry (J Microsc 6:987-1018, 1967), in the principal regions (II, III, IV). Electron tomography analysis of the free flagellum and one axoneme of a mature spermatozoon of C. fennica provides clear evidence, for the first time, that two tubular structures are present in the central axonemal electron-dense core. Phylogenetically important aspects of spermiogenesis of the Caryophyllidea with one axoneme, and other cestodes with one or two axonemes, are briefly reviewed and discussed.
Faculty of Science University of South Bohemia České Budějovice Czech Republic
Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 040 01 Košice Slovak Republic
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