Biomaterial for tissue engineering is a topic of huge progress with a recent surge in fabrication and characterization advances. Biomaterials for tissue engineering applications or as scaffolds depend on various parameters such as fabrication technology, porosity, pore size, mechanical strength, and surface available for cell attachment. To serve the function of the scaffold, the porous biomaterial should have enough mechanical strength to aid in tissue engineering. With a new manufacturing technology, we have obtained high strength materials by optimizing a few processing parameters such as pressure, temperature, and dwell time, yielding the monolith with porosity in the range of 80%⁻93%. The three-dimensional interconnectivity of the porous media through scales for the newly manufactured biomaterial has been investigated using newly developed 3D correlative and multi-modal imaging techniques. Multiscale X-ray tomography, FIB-SEM Slice & View stacking, and high-resolution STEM-EDS electronic tomography observations have been combined allowing quantification of morphological and geometrical spatial distributions of the multiscale porous network through length scales spanning from tens of microns to less than a nanometer. The spatial distribution of the wall thickness has also been investigated and its possible relationship with pore connectivity and size distribution has been studied.

CNRS Univ Bordeaux ICMCB UMR 5026 F 33600 Pessac France

Thermo Fisher Scientific Impasse Rudolf Diesel 33700 Merignac France

Thermo Fisher Scientific Vlastimila Pecha 1282 12 62700 Brno Czech Republic

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Ratner B.D., Hoffman A.S., Schoen F.J., Lemons J.E. Biomaterials Science–An Introduction to Materials in Medicine. Elsevier Academic Press; San Diego, CA, USA: 2004.

Anderson D.G., Burdick J.A., Langer R. Smart biomaterials. Science. 2004;305:1923–1924. doi: 10.1126/science.1099987. PubMed DOI

Alvarez K., Nakajima H. Metallic scaffolds for bone regeneration. Materials. 2009;2:790–832. doi: 10.3390/ma2030790. DOI

Li Z., Gu X.N., Lou S., Zheng Y.F. The development of binary Mg–Ca alloys for use as biodegradable materials within bone. Biomaterials. 2008;29:1329–1344. doi: 10.1016/j.biomaterials.2007.12.021. PubMed DOI

Wu S.L., Liu X.M., Yeung K.W.K., Hu T., Xu Z.S., Chung C.Y., Chu P.K. Hydrogen release from titanium hydride in foaming of orthopedic NiTi scaffolds. Acta Biomater. 2011;7:1387–1397. doi: 10.1016/j.actbio.2010.10.008. PubMed DOI

He J., Huang T., Gan L., Zhou Z.K., Jiang B., Wu Y., Wu F., Gu Z.W. Collagen-infiltrated porous hydroxyapatite coating and its osteogenic properties: In vitro and in vivo study. J. Biomed. Mater. Res. Part A. 2012;100:1706–1715. doi: 10.1002/jbm.a.34121. PubMed DOI

Weiner S., Wagner H.D. The material bone: Structure-mechanical function relations. Annu. Rev. Mater. Sci. 1998;28:271–298. doi: 10.1146/annurev.matsci.28.1.271. DOI

LeGeros R.Z. Properties of osteoconductive biomaterials: Calcium Phosphates. Clin. Orthop. Relat. Res. 2002;395:81–98. doi: 10.1097/00003086-200202000-00009. PubMed DOI

Gauthier O., Bouler J.M., Aguado E., Pilet P., Daculsi G. Macroporous biphasic Calcium Phosphate ceramics: Influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials. 1998;19:133–139. doi: 10.1016/S0142-9612(97)00180-4. PubMed DOI

Davies J.E. In vitro modeling of the bone/implant interface. Anat. Rec. 1996;245:426–445. doi: 10.1002/(SICI)1097-0185(199606)245:2<426::AID-AR21>3.0.CO;2-Q. PubMed DOI

Anselme K. Osteoblast adhesion on biomaterials. Biomaterials. 2000;21:667–681. doi: 10.1016/S0142-9612(99)00242-2. PubMed DOI

Prakasam M., Locs J., Salma-Ancane K., Loca D., Largeteau A., Berzina-Cimdina L. Fabrication, Properties and Applications of Dense Hydroxyapatite: A Review. J. Funct. Biomater. 2015;6:1099–1140. doi: 10.3390/jfb6041099. PubMed DOI PMC

Eksi A., Saritas S. Effects of Powder Hardness and Particle Size on the Densification of Cold Isostatically Pressed Powders. Turk. J. Eng. Environ. Sci. 2002;26:377–384.

Byrappa K., Yoshimura M. Handbook of Hydrothermal Technology. William Andrew; Norwich, NY, USA: 2001. A Technology for Crystal Growth and Materials Processing.

Bouville F., Studart A.R. Geologically-inspired strong bulk ceramics made with water at room temperature. Nat. Commun. 2017;8:14655. doi: 10.1038/ncomms14655. PubMed DOI PMC

Ndayishimiye A., Largeteau A., Prakasam M., Pechev S., Dourges M.-A., Goglio G. Low temperature hydrothermal sintering process for the quasi-complete densification of nanometric α-quartz. Scr. Mater. 2018;145:118–121. doi: 10.1016/j.scriptamat.2017.10.023. DOI

Blackford J.R. Sintering and microstructure of ice: A review. J. Phys. D Appl. Phys. 2007;40:R355–R385. doi: 10.1088/0022-3727/40/21/R02. DOI

Le Bail A., Chevalier D., Mussa D.M., Ghoul M. High pressure freezing and thawing of foods: A review. Int. J. Refrig. 2002;25:504–513. doi: 10.1016/S0140-7007(01)00030-5. DOI

Largeteau A., Prakasam M. Trends in high pressure developments for new perspectives. Solid State Sci. 2018;80:141–146. doi: 10.1016/j.solidstatesciences.2018.04.012. DOI