A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples

. 2018 ; 147 () : 59-75. [epub] 20180729

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid30165963
Odkazy

PubMed 30165963
DOI 10.1016/bs.mcb.2018.07.001
PII: S0091-679X(18)30088-8
Knihovny.cz E-zdroje

Bacterial contamination and subsequent infections are a major threat to human health. An early detection in the food chain, clinics or the environment, is key to limit this threat. We present a new concept to develop low-cost hand-held devices for the ultra-sensitive and specific detection of bacteria in a one-step process of 2-8h, directly from complex raw samples. This approach is based on a novel microfluidic magnetic fluidized bed. It reaches a 4CFU (colony forming unit) sensitivity with high quantification accuracy in a large dynamic range of 100-107CFU/mL. The versatility of the approach was demonstrated with the detection of different bacteria strains, among which Salmonella Typhimurium and E. coli O157:H15. Additionally, the method is sensitive to infectious bacteria only, a criterion requested by main applications and currently requiring additional culture steps of one to several days.

Citace poskytuje Crossref.org

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