A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30165963
DOI
10.1016/bs.mcb.2018.07.001
PII: S0091-679X(18)30088-8
Knihovny.cz E-zdroje
- Klíčová slova
- Amplification, Bacteria, Capture, Detection, Fluidized bed, Magnetic beads,
- MeSH
- antibakteriální látky farmakologie MeSH
- mikrofluidika metody MeSH
- počítačové zpracování obrazu MeSH
- Salmonella typhimurium účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
Bacterial contamination and subsequent infections are a major threat to human health. An early detection in the food chain, clinics or the environment, is key to limit this threat. We present a new concept to develop low-cost hand-held devices for the ultra-sensitive and specific detection of bacteria in a one-step process of 2-8h, directly from complex raw samples. This approach is based on a novel microfluidic magnetic fluidized bed. It reaches a 4CFU (colony forming unit) sensitivity with high quantification accuracy in a large dynamic range of 100-107CFU/mL. The versatility of the approach was demonstrated with the detection of different bacteria strains, among which Salmonella Typhimurium and E. coli O157:H15. Additionally, the method is sensitive to infectious bacteria only, a criterion requested by main applications and currently requiring additional culture steps of one to several days.
Citace poskytuje Crossref.org