Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"
Language English Country United States Media print
Document type Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't, Comment
Grant support
646451
European Research Council - International
R01 NS056114
NINDS NIH HHS - United States
PubMed
30166461
PubMed Central
PMC7611747
DOI
10.1126/science.aau8230
PII: 361/6405/eaau8230
Knihovny.cz E-resources
- MeSH
- X-Ray Diffraction * MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Protein Conformation MeSH
- Scattering, Small Angle * MeSH
- Intrinsically Disordered Proteins MeSH
- Water MeSH
- Publication type
- Journal Article MeSH
- Comment MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Intramural MeSH
- Names of Substances
- Intrinsically Disordered Proteins MeSH
- Water MeSH
Editors at Science requested our input on the above discussion (comment by Best et al and response by Riback et al) because both sets of authors use our data from Fuertes et al (2017) to support their arguments. The topic of discussion pertains to the discrepant inferences drawn from SAXS versus FRET measurements regarding the dimensions of intrinsically disordered proteins (IDPs) in aqueous solvents. Using SAXS measurements on labeled and unlabeled proteins, we ruled out the labels used for FRET measurements as the cause of discrepant inferences between the two methods. Instead, we propose that FRET and SAXS provide complementary readouts because of a decoupling of size and shape fluctuations that is intrinsic to finite-sized, heteropolymeric IDPs. Accounting for this decoupling resolves the discrepant inferences between the two methods, thus making a case for the utility of both methods.
European Molecular Biology Laboratory 22607 Hamburg Germany
European Molecular Biology Laboratory 69117 Heidelberg Germany
See more in PubMed
Fuertes G, et al. Proc Natl Acad Sci USA. 2017;114:E6342–E6351. PubMed PMC
Riback JA, et al. Science. 2017;358:238–241. PubMed PMC
Borgia A, et al. J Am Chem Soc. 2016;138:11714–11726. PubMed PMC
Riback JA, et al. Science. 2018;361:eaar7949. PubMed
Best RB, et al. Science. 2018;361:eaar7101. PubMed
O’Brien EP, Morrison G, Brooks BR, Thirumalai D. J Chem Phys. 2009;130:124903. PubMed PMC
Song J, Gomes GN, Shi T, Gradinaru CC, Chan HS. Biophys J. 2017;113:1012–1024. PubMed PMC
Watkins HM, et al. Proc Natl Acad Sci USA. 2015;112:6631–6636. PubMed PMC
Valentini E, Kikhney AG, Previtali G, Jeffries CM, Svergun DI. Nucleic Acids Res. 2015;43:D357–D363. PubMed PMC
