The population of California sea lion Zalophus californianus (CSL) has steadily increased during the last several decades. Despite extensive research addressing CSL biology and ecology performed during the last decades, there has been a minimal number of published papers documenting their parasite fauna. Our objective was to analyze the actual list of the metazoan parasites reported from CSLs and add new data on the age-related differences in the prevalence and biodiversity of the parasite community. There have been 33 species recorded but this study considers only 24 of them valid. Among them, 11 species are specific parasites of CSLs and 13 species are not specific. Additional species represent accidental infections or misidentifications. In total, 6653 helminths and 847 mites were collected and identified from 34 CSLs for this study. Six species of nematodes, Anisakis simplex sensu lato s. l. (prevalence 41%; intensity 7.6), Contracaecum ogmorhini s. l. (38%; 269.6), Pseudoterranova decipiens s. l. (29%; 33), P. azarazi (9%; 2.7), Acanthocheilonema odendhali (15%; 3.5) and Parafilaroides decorus were found. Two species of cestodes, Diphyllobothrium sp. (38%; 8.5) and Anophryocephalus sp. (15%; 14.6) represent novel undescribed species. Two species of trematodes, Apophallus zalophi (18%; 19.7) and Zalophotrema hepaticum (12%; 39.2), and five species of acanthocephalans, Corynosoma obtuscens (68%; 100.8), C. strumosum (53%; 4.6), Andracantha phalacrocoracis (3%; 1), Andracantha sp. (9%; 4.3) and Profilicollis altmani (6%; 8.5) were found. Mites Orthohalarchne attenuata (prevalence 85%) were found in the nasal cavity, while O. diminuata (21%) parasitized in the trachea and bronchi. The highest levels of infection with nematodes and trematodes were found in adult CSLs (3-16 years old), whereas the highest level of infection with acanthocephalans was found in young CSLs (pups and yearlings).

1 I Schmalhausen Institute of Zoology NAS of Ukraine 15 Bogdan Khmelnitsky Street Kyiv 01030 Ukraine

Colorado State University Diagnostic Laboratory Department of Microbiology Immunology and Pathology College of Veterinary Medicine and Biomedical Sciences Colorado State University Fort Collins CO 80526 USA

Institute of Ecology Nature Research Centre Akademijos 2 08412 Vilnius Lithuania

Institute of Parasitology Biology Centre of the Czech Academy of Sciences Branišovská 31 37005 České Budějovice Czech Republic

The Marine Mammal Center 2000 Bunker Road Fort Cronkhite Sausalito CA 94965 2619 USA

Water Research Group Unit for Environmental Sciences and Management Potchefstroom Campus North West University Potchefstroom 2520 South Africa

Witold Stefański Institute of Parasitology of Polish Academy of Sciences; Twarda Street 51 55 00 818 Warsaw Poland

Zobrazit více v PubMed

Adell A.D., Smith W.A., Shapiro K., Melli A., Conrad P.A. Molecular epidemiology of Cryptosporidium spp. and Giardia spp. in mussels (Mytilus californianus) and California sea lions (Zalophus californianus) from Central California. Appl. Environ. Microbiol. 2014;80:7732–7740. PubMed PMC

Alho A.M., Marcelino I., Colella V., Flanagan C., Silva N., Correia J.J., Latrofa M.S., Otranto D., Madeira de Carvalho L. Dirofilaria immitis in pinnipeds and new host record. Parasit. Vect. 2017;10:142. PubMed PMC

Ashizawa H., Ezaki T., Arie Y., Nosaka D., Tateyama S., Owada K. Pathological findings on lungworm disease in California sea lions. Bull. Fac. Agric. Miyazaki Univ. 1978;25:287–295.

Carlson-Bremer D., Colegrove K.M., Gulland F.M., Conrad P.A., Mazet J.A., Johnson C.K. Epidemiology and pathology of Toxoplasma gondii in free-ranging California sea lions (Zalophus californianus) J. Wildl. Dis. 2015;51:362–373. PubMed

Carlson-Bremer D., Johnson C.K., Miller R.H., Gulland F.M., Conrad P.A., Wasmuth J.D., Colegrove K.M., Grigg M.E. Identification of two novel coccidian species shed by California sea lions (Zalophus californianus) J. Parasitol. 2012;98:347–354. PubMed PMC

Carretta J.V., Forney K.A., Lowry M.S., Barlow J., Baker J., Hanson B., Muto M.M. US Department of Commerce; 2007. U.S. Pacific Marine Mammal Stock Assessments: 2007. NOAA Technical Memorandum, NMFS–SWFSC–414.

Ciurea I. Les vers parasites de l'homme, des mammifères et des oiseaux provenant des Poissons du Danube et de la mer Noire. Arch. Roumaines Pathol. Exp. Microbiol. 1933;6:5–135.

Dahme E., Popp E. Todesfälle bei Seelöwen (Zalophus californianus Lesson) verursacht durch eine bisher unbekannte Milbe (Orthohalarachne letalis Popp) Berl. Münchener Tierärztliche Wochenschr. 1963;21:441–443.

Dailey M.D. Stictodora ubelakeri a new species of heterophyid trematode from the California sea lion (Zalophus californianus) Bull. South Calif. Acad. Sci. 1969;68:82–85.

Dailey M.D. The transmission of Paraflaroides decorus (Nematode: Metastrongyloidae) in the California sea lion (Zalophus californianus) Proc. Helminthol. Soc. Wash. 1970;37:215–222.

Dailey M.D. The distribution and intraspecific variation of helminth parasites in pinnipeds. Rapp. P.-V. Reun. Cons. Int. Explor. Mer. 1975;169:338–352.

Dailey M.D., Brownell R.L.J. A checklist of mammal parasites. In: Ridgeway S.H., Springfield C.C.T., editors. Mammals of the Sea: Biology and Medicine. Thomas; Springfield: 1972. pp. 528–589.

Dailey M.D. vol. III. 1972. Baseline data on parasites from marine mammals in Southern California; pp. 442–472. (Marine Mammals and Seabird Survey of the Southern California Bight Area). Principal investigator’s reports. Book 1. Pinnipedia, Cetacea and Parasitology.

Dailey M.S., Ellin R., Parás A. First report of parasites from pinnipeds in the Galapagos Islands, Ecuador, with a description of a new species of Philophthalmus (Digenea: Philophthalmidae) J. Parasitol. 2005;91:614–617. PubMed

Dailey M.D., Hill B.L. A survey of metazoan parasites infesting the California (Zalophus californianus) and Steller (Eumetopias jubatus) sea lion. Bull. South Calif. Acad. Sci. 1970;69:126–132.

Dailey M.D., Nutting W.B. Demodex zalophi sp. nov. (Acari: Demodicidae) from Zalophus californianus, the California sea lion. Acarologia. 1980;21:423–428. PubMed

D'Amelio S., Mattiucci S., Paggi L., Orecchia P., Nascetti G., Cianchi R., Bullini L., Margolis L. Genetic variation of the pinniped parasite Contracaecum ogmorhini (Ascaridida, Anisakidae) Parassitologia. 1994;36(Suppl. l):40.

Delyamure S.L. Izdat. Ak. Nauk SSSR; Moscow, Russia: 1955. Helminthofauna of Marine Mammals (Ecology and Phylogeny) p. 517. (in Russian)

Delyamure S.L., Skryabin A.S., Serdiukov A.M. Diphyllobothriata—flatworm parasites of man, mammals and birds. In: Ponova T.I., Sonc M.D., editors. Principles of Cestodology. vol. 9. Nauka; Moscow, Russia: 1985. p. 200. (in Russian)

Doetschman W.H. A new species of endoparasitic mite of the family Halarachnidae (Acarina) Trans. Am. Microsc. Soc. 1944;63:68–72.

Dougherty E.C., Herman C.M. New species of the genus Parafilaroides Dougherty, 1946 (Nematoda: Metastrongylidae), from sea-lions, with a list of the lungworms of the pinnipedia. Proc. Helminthol. Soc. Wash. 1947;14:77–87. PubMed

Ezzat M.A.E., Tardos G., El Halim M.I.A. Human schistosomes and other trematodes in sea-lions, Zalophus californianus from Giza Zoological Gardens. Bull. Min. Agric. Egypt Vet. Lab. Res. Admin. 1958;285:1–20.

Fagerholm H.P., Gibson D.I. A redescription of the pinniped parasite Contracaecum ogmorhini (Nematoda, Ascaridoidea), with an assessment of its antiboreal circumpolar distribution. Zool. Scripta. 1987;16:19–24.

Faust E.C. Mammalian heart worms of the genus Dirofilaria. In: Benesch F., editor. Festschrift, Bernhard Nocht zum 80 Geburtstag von seinen Freunden und Schulern. Friederichsen; Hamburg, Germany: 1937. pp. 131–137.

Felix J.R. Zea E-Books; 2013. Reported Incidences of Parasitic Infections in Marine Mammals from 1892 to 1978. Book 20.

Ferris G.F. Anoplura from sea lions of the Pacific ocean. Entomol. News. 1916;27:366–370.

Ferris G.F. vol. 2. Part VII. Stanford Univ. PubIs, Univ; 1934. Contributions toward a Monograph of the Sucking Lice; pp. 471–526. Ser. (BioI. Sci.)

Ferris G.F. The sucking lice. Mem. Pacific. Coast Ent. Soc. 1951;1:1–320.

Fleischman R.W., Squires R.A. Verminous pneumonia in the California sea lion (Zalophus californianus) Pathol. Vet. 1970;7:89–101. PubMed

Flores-Barroeta L., Hidalgo-Escalante E., Oleac R. Nematodes from birds and mammals. IV (1). Erratic parasitosis in Zalophus californianus from Asuncion Island, Baja California, Mexico. Helminthologia. 1961;3:112–116.

Furman D.P. Observations on the ontogeny of halarachnid mites (Acarina: Halarachnidae) J. Parasitol. 1977;63:748–755. PubMed

Gaevskaya A.V. Anisakid nematodes and diseases caused by them in animals and man. Sevastopol: EKOSI–Gidrofizika. 2005;1–223 (In Russian)

Geraci J.R. The enigma of marine mammal strandings. Oceanus. 1978;21:38–47.

Girard Y.A., Johnson C.K., Fritz H.M., Shapiro K., Packham A.E., Melli A.C., Carlson-Bremer D., Gulland F.M., Rejmanek D., Conrad P.A. Detection and characterization of diverse coccidian protozoa shed by California sea lions. Int. J. Parasitol. Parasites Wildl. 2015;5:5–16. PubMed PMC

Hammer Ø., Harper D.A.T., Ryan P.D. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Paleontol. Electron. 2001;4(1):9pp. http://palaeo-electronica.org/2001_1/past/issue1_01.htm

Herman C.M. The effect of higueronia on nematodes and nemathelmintic gastric ulcers of California sea lions. Rev. Med. Trop. Parasitol. Bacterid. Clinica y Lab. 1942;8:45–47.

Hernández-Orts J.S., Scholz T., Brabec J., Kuzmina T., Kuchta R. Unexpected morphological plasticity and global geographical distribution of the Pacific human tapeworm Adenocephalus pacificus (syn. Diphyllobothrium pacificum): molecular and morphological survey. Acta Trop. 2015;149:168–178. PubMed

Hernández-Orts J.S., Scholz T., Brabec J., Kuzmina T., Kuchta R. Does the number of genital organs matter? Case of the seal tapeworm Diphyllobothrium (syn. Diplogonoporus) tetrapterum (Cestoda: Diphyllobothriidea) Can. J. Zool. 2018;96:193–204.

Jellison W.L. Anoplura from mammals of the Pribilof Islands. J. Parasitol. 1952;38:274–275.

Johnson D.G., Ridgway S.H. Parasitism in some marine mammals. J. Am. Vet. Med. Assoc. 1969;155:1064–1072. PubMed

Kagei N., Oda T. Dipetalonema odendhali Perry, 1967 found from sur seal, Callorhinus ursinus (Linnaeus, 1758) Bull. Inst. Publ. Hlth. 1975;24:203–205. (in Japanese)

Kelly T.R., Greig D., Colegrove K.M., Lowenstine L.J., Dailey M., Gulland F.M., Haulena M. Metastrongyloid nematode (Otostrongylus circumlitus) infection in a stranded California sea lion (Zalophus californianus) – a new host-parasite association. J. Wildl. Dis. 2005;41:593–598. PubMed

King J.E. Seals of the world. London. Br. Mus. Nat. Hist. 1964:1–151.

Kinne O. Diseases of marine animals. Volume IV, Part 2, introduction, reptilia, Aves, Mammalia. Biol. Anst. Helgol. Hamburg. 1985;1–884

Kuchta R., Scholz T. Diphyllobothriidea. In: Caira J.N., Jensen K., editors. Planetary Biodiversity Inventory (2008–2017): Tapeworms from Vertebrate Bowels of the Earth. University of Kansas, Natural History Museum; Lawrence, KS, USA: 2017. pp. 167–189. Special Publication No. 25.

Kuzmina T.A., Hernández-Orts J.S., Lyons E.T., Spraker T.R., Kornyushyn V.V., Kuchta R. Cestode community in northern Fur seals (Callorhinus ursinus) on St. Paul Island, Alaska. Int. J. Parasitol. Parasites Wildl. 2015;4:256–263. PubMed PMC

Kuzmina T.A., Kuzmin Y. Description of Uncinaria lyonsi n. sp. (Nematoda: Ancylostomatidae) from the California sea lion Zalophus californianus Lesson (Carnivora: Otariidae) Syst. Parasitol. 2015;90:165–176. PubMed

Kuzmina T.A., Kuzmin Y.I., Tkach V.V., Spraker T.R., Lyons E.T. Ecological, morphological, and molecular studies of Acanthocheilonema odendhali (Nematoda: Filarioidea) in northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska. Parasitol. Res. 2013;112:3091–3100. PubMed

Kuzmina T.A., Lisitsyna O.I., Lyons E.T., Spraker T.R., Tolliver S.C. Acanthocephalans in northern Fur seals (Callorhinus ursinus) and a harbor seal (Phoca vitulina) on St. Paul Island, Alaska: species, prevalence and biodiversity in four fur seal subpopulations. Parasitol. Res. 2012;111:1049–1058. PubMed

Kuzmina T.A., Lyons E.T., Spraker T.R. Anisakids (Nematoda: Anisakidae) from stomachs of northern Fur seals (Callorhinus ursinus) on St. Paul Island, Alaska: parasitological and pathological analysis. Parasitol. Res. 2014;113:4463–4470. PubMed

Kuzmina T.A., Tkach V.V., Spraker T.R., Lyons E.T., Kudlai O. Digeneans of northern fur seals Callorhinus ursinus (Pinnipedia: Otariidae) from five subpopulations on St. Paul Island, Alaska. Parasitol. Res. 2018;117:1079–1086. PubMed

Laake J.L., Melin S.R., Orr A.J., Greig D.J., Prager K.C., DeLong R.L., Harris J.D. U.S. Dep. Commer., NOAA Tech. Memo; 2016. California Sea Lion Sex- and Age-specific Morphometry. NMFS–AFSC–312.

Lefoulon E., Bain O., Bourret J., Junker K., Guerrero R., Canizales I., Kuzmin Y., Satoto T.B.T., Cardenas-Callirgos J.M., de Souza Lima S., Raccurt C., Mutafchiev Y., Gavotte L., Martin C. Shaking the tree: multi-locus sequence typing usurps current onchocercid (filarial nematode) phylogeny. PLoS Neglected Trop. Dis. 2015;9:E0004233. PubMed PMC

Lincicome D.R. Acanthocephala of the genus Corynosoma from the California sea lion. J. Parasitol. 1943;29:102–106.

Lowry M.S., Stewart B.S., Heath C.B., Yochem P.K., Francis J.M. Seasonal and annual variability in the diet of California sea lions Zalophus californianus at San Nicholas Island, California, 1981–86. Fish. Bull. 1991;89:331–336.

Lyons E.T., DeLong R.L., Gulland F.M., Melin S.R., Tolliver S.C., Spraker T.R. Comparative biology of Uncinaria spp. in the California sea lion (Zalophus californianus) and the northern fur seal (Callorhinus ursinus) in California. J. Parasitol. 2000;86:1348–1352. PubMed

Lyons E.T., DeLong R.L., Melin S.R., Tolliver S.C. Uncinariasis in northern fur seal and California sea lion pups from California. J. Wildl. Dis. 1997;33:848–852. PubMed

Lyons E.T., DeLong R.L., Nadler S.A., Laake J.L., Orr A.J., DeLong B.L., Pagan C. Investigations of peritoneal and intestinal infections of adult hookworms (Uncinaria spp.) in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California (2003) Parasitol. Res. 2011;109:581–589. PubMed

Lyons E.T., Kuzmina T.A., Spraker T.R., Melin S.R., DeLong R. Hookworms (Uncinaria lyonsi) in dead California sea lion (Zalophus californianus) pups and sand in winter (2014–2015) on San Miguel Island, California. Comp. Parasitol. 2016;83:283–286.

Marcogliese D.J. Food webs and the transmission of parasites to marine fish. Parasitology. 2002;124:S83–S99. PubMed

Marcogliese D.J., Cone D.K. Food webs: a plea for parasites. Trends Ecol. Evol. 1997;12:320–325. PubMed

Margolis L. Parasitic helminths and arthropods from pinnipedia of the Canadian Pacific coast. J. Fish. Res. Board Can. 1956;13:489–505.

Margolis L. List of the parasites recorded from sea mammals caught off the West coast of North America. J. Fish. Res. Board. Can. 1954;11:267–283.

Margolis L., Dailey M.D. 1972. Revised Annotated List of Parasites from Sea Mammals Caught of the West Coast of North America; pp. 1–23. NOAA Technical report NMFS, SSRF-647.

Mariaux J., Kuchta R., Hoberg E.P. Tetrabothriidea baer, 1954. In: Caira J.N., Jensen K., editors. Planetary Biodiversity Inventory (2008–2017): Tapeworms from Vertebrate Bowels of the Earth. University of Kansas, Natural History Museum; Lawrence, KS, USA: 2017. pp. 357–370. Special Publication No. 25.

Mattiucci S., Cianchi R., Nascetti G., Paggi L., Sardella N., Timi J., Webb S.C., Bastida R., Rodriguez D., Bullini L. Genetic evidence for two sibling species within Contracaecum ogmorhini Johnston and Mawson (1941) (Nematoda: Anisakidae) from otariid seals of Boreal and Austral regions. Syst. Parasitol. 2003;54:13–23. PubMed

Mattiucci S., Nascetti G. Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Adv. Parasitol. 2008;66:47–148. PubMed

McClatchie S., Field J., Thompson A.R., Gerrodette T., Lowry M., Fiedler P.C., Watson W., Nieto K.M., Vetter R.D. Food limitation of sea lion pups and the decline of forage off central and southern California. Royal Soc. Open Sci. 2016;3:150628. PubMed PMC

McClelland G. The trouble with sealworms (Pseudoterranova decipiens species complex, Nematoda): a review. Parasitology. 2002;124:183–203. PubMed

McClelland G., Misra G.R.K., Martell D.J. Larval anisakine nematodes in various fish species from Sable Island Bank and vicinity. Can. Bull. Fish. Aquat. Sci. 1990;222:83–113.

Nagasawa K. Parasites of pinniped (Mammalia: Carnivora) in Japan: checklist and bibliography. Bull. Nat. Res. Inst. Far Seas Fish. 1999;1999:27–32.

Newell I.M. Studies on the morphology and systematics of the family Halarachnidae Oudemans, 1906 (Acari, Parasitoidea) B. Bingham Oceanogr. C. 1947;10:235–266.

Paggi L., Nascetti G., Cianchi R., Orecchia P., Mattiucci S., D'Amelio S., Berland B., Brattey J., Smith J.W., Bullini L. Genetic evidence for three species within Pseudoterranova decipiens (Nematoda, Ascaridida, Ascaridoidea) in the North Atlantic and Norwegian and barents seas. Int. J. Parasitol. 1991;21:195–212. PubMed

Perry M.L. A new species of Dipetalonema from the California sea lion and a report of microflariae from a Steller sea lion (Nematoda: Filaroidea) J. Parasitol. 1967;53:1076–1081. PubMed

Perry M.L., Forrester D.J. Dipetalonema odendhali (Nematoda: Filarioidea) from the northern fur seal, with a description of the microfilaria. J. Parasitol. 1971;57:469–472. PubMed

Poulin R., Chappell L.H. Cambridge University Press; 2004. Parasites in Marine Systems; p. 216 pp.

Price E.W. The trematode parasites of marine mammals. Proc. U. S. Natl. Mus. 1932;81:1–67.

Ridgway S.H., Geraci J.R., Medway W. Diseases of pinnipeds. Rapp. P.-V. Reun. Cons. Int. Explor. Mer. 1975;169:327–337.

Sato T., Higuchi T., Shibuya H., Ohba S., Nogami S., Shirai W., Watanabe H., Honda S. Lingual squamous cell carcinoma in a California sea lion (Zalophus californianus) J. Zoo Wildl. Med. 2002;33:367–370. PubMed

Schroeder C.R., Wegeforth H.M. The occurrence of gastric ulcers in sea mammals of the California coast, their etiology and pathology. J. Am. Vet. Med. Assoc. 1935;87:333–342.

Schroeder R.J., Delli Quadri C.A., McIntyre R.W., Walker W.A. Marine mammal disease surveillance program in Los Angeles County. J. Am. Vet. Med. Assoc. 1973;163:580–581. PubMed

Shamsi S., Norman R., Gasser R., Beveridge I. Redescription and genetic characterization of selected Contracaecum spp. (Nematoda: Anisakidae) from various hosts in Australia. Parasitol. Res. 2009;104:1507–1525. PubMed

Smith J.W., Wootten R. Anisakis and anisakiasis. Adv. Parasitol. 1978;16:93–163. PubMed

Spraker T.R., Lyons E.T., DeLong R.L., Zink R.R. Penetration of the small intestine of a California sea lion (Zalophus californianus) pup by adult hookworms (Uncinaria spp) Parasitol. Res. 2004;92:436–438. PubMed

Spraker T.R. Basic necropsy procedures. In: McCurnin D.M., editor. Clinical Textbook for the Animal Health Technician. W.B. Saunders Company; London, UK: 1985. pp. 4702–4816.

Stroud R.K., Dailey M.D. Parasites and associated pathology observed in pinnipeds stranded along the Oregon coast. J. Wildl. Dis. 1978;14:292–298. PubMed

Stunkard H.W. Variation and criteria for generic and specific determination of diphyllobothriid cestodes. J. Helminthol. 1965;39:281–296. PubMed

Stunkard H.W., Alvey C.H. A new liver fluke, Zalophotrema hepaticum, from the California sea lion, Zalophus californianus. J. Parasitol. 1929;16:106.

Stunkard H.W., Alvey C.H. The morphology of Zalophotrema hepaticum with a review of the trematode family Fasciolidae. Parasitology. 1930;22:326–333.

Sweeney J.C. Management of pinniped diseases. Am. Ass. Zoo. Vet. An. Proc. 1973;1973:141–171.

Sweeney J.C., Gilmartin W.G. Survey of diseases in free-living California sea lions. J. Wildl. Dis. 1974;10:370–376. PubMed

Taylor A.E.R., Brown D.H., Heyneman D., McIntyre R.W. Biology of filarioid nematode Dipetalonema spirocauda (Leidy, 1858) from the heart of captive harbor seals and sea lions, together with pathology of the hosts. J. Parasitol. 1961;47:971–976. PubMed

Thompson R.M., Mouritsen K.N., Poulin R. Importance of parasites and their life cycle characteristics in determining the structure of a large marine food web. J. Anim. Ecol. 2005;74:77–85.

Van Cleave H.J. A preliminary analysis of the acanthocephalan genus Corynosoma in mammals of North America. J. Parasitol. 1953;39:1–13. PubMed

Waeschenbach A., Brabec J., Scholz T., Littlewood D.T.J., Kuchta R. The catholic taste of broad tapeworms – multiple routes to human infection. Int. J. Parasitol. 2017;47:831–843. PubMed

White G.L. Dirofilaria immitis and heartworm disease in the California sea lion. J. Zoo Anim. Med. 1975;6:23–24.

Yamaguti S. Interscience Publishers; New York & London: 1961. Systema Helminthum. Vol. III. The Nematodes of Vertebrates, Pt. II & I. 1261 pp.

Zhu X., D'Amelio S., Hu M., Paggi L., Gasser R. Electrophoretic detection of population variation within Contracaecum ogmorhini (Nematoda: Ascaridoidea: Anisakidae) Electrophoresis. 2001;22:1930–1934. PubMed

Diphyllobothrium sprakeri n. sp. (Cestoda: Diphyllobothriidae): a hidden broad tapeworm from sea lions off North and South America

Broad tapeworms (Diphyllobothriidae), parasites of wildlife and humans: Recent progress and future challenges