PURPOSE: The aim of this study was to investigate the influence of the cardiorespiratory fitness level on the response to high-intensity interval training (HIIT) with an individually adjusted running speed of the same relative intensity. The evaluation focused on acute cardiorespiratory response, postexercise cardiac autonomic modulation (heart rate variability (HRV)) and biochemical markers of inflammation, oxidative stress, and muscle damage. METHODS: Thirty participants were divided into 3 subgroups: well trained, moderately trained, and untrained. All the participants performed 30 min HIIT composed of 6 × 2 min interval exercise with work-to-relief ratio = 1 and work intensity 100% of individual velocity at maximal oxygen consumption (VO2​max ). Acute cardiorespiratory variables, postexercise HRV, lactate, interleukin-6 (IL-6), total antioxidant capacity (TAC), creatine kinase, and myoglobin up to 4 h after HIIT were monitored. RESULTS: The differences in relatively expressed cardiorespiratory variables (heart rate, VO2) during HIIT were at most moderate, with the most pronounced between-group differences in absolute VO2 values. The disruption of the postexercise HRV was the most pronounced in untrained individuals, and this difference persisted 1 h after HIIT. The highest postexercise IL-6 and TAC concentrations and the lowest changes in creatine kinase and myoglobin were revealed in well-trained individuals. CONCLUSION: The higher fitness level was associated with the less pronounced postexercise cardiac autonomic changes and their faster restoration, even when there were similar acute cardiorespiratory responses. These findings were simultaneously accompanied by the higher postexercise IL-6 and TAC concentrations and less significant changes in muscle damage biochemical markers in well-trained individuals.

Human Motion Diagnostic Centre and Department of Human Movement Studies Ostrava University Ostrava 702 00 Czech Republic

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Baechle T.R., Earle R.W. Human Kinetics; Champaign, IL: 2008. Essentials of strength training and conditioning.

Milanović Z., Sporiš G., Weston M. Effectiveness of high-intensity interval training (HIT) and continuous endurance training for VO2max improvements: a systematic review and meta-analysis of controlled trials. Sports Med. 2015;45:1469–1481. PubMed

Buchheit M., Laursen P.B. High-intensity interval training, solutions to the programming puzzle—part I: cardiopulmonary emphasis. Sports Med. 2013;43:313–338. PubMed

Stuckey M.I., Tordi N., Mourot L., Gurr L.J., Rakobowchuk M., Millar P.J. Autonomic recovery following sprint interval exercise. Scand J Med Sci Sports. 2012;22:756–763. PubMed

Buchheit M. Monitoring training status with HR measures: do all roads lead to Rome? Front Physiol. 2014;5:1–9. PubMed PMC

Kaikkonen P., Rusko H., Martinmäki K. Post-exercise heart rate variability of endurance athletes after different high-intensity exercise interventions. Scand J Med Sci Sports. 2008;18:511–519. PubMed

Kiviniemi A.M., Tulppo M.P., Eskelinen J.J., Savolainen A.M., Kapanen J., Heinonen I.H. Cardiac autonomic function and high-intensity interval training in middle-aged men. Med Sci Sports Exerc. 2014;46:1960–1967. PubMed

Pedersen B.K., Febbraio M.A. Muscle, exercise and obesity: skeletal muscle as secretory organ. Nat Rev Endocrinol. 2012;8:457–465. PubMed

Raschke S., Eckel J. Adipo-myokines: two sides of the same coin—mediators of inflammation and mediators of exercise. Mediators Inflamm. 2013;2013:320724. PubMed PMC

Wadley A.J., Chen Y.W., Lip G.Y., Fisher J.P., Aldred S. Low-volume-high intensity interval exercise elicits antioxidant and anti-inflammatory effects in humans. J Sports Sci. 2016;34:1–9. PubMed

Zweetsloot K.A., John C.S., Lawrence M.M., Battista R.A., Shanely R.A. High-intensity interval training induces a modest systemic inflammatory response in active, young men. J Inflamm Res. 2014;7:9–17. PubMed PMC

Valko M., Leibfritz D., Moncol J., Cronin M.T., Mazur M., Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39:44–84. PubMed

Pingitore A., Lima G.P., Mastorci F., Quinones A., Iervasi G., Vassalle C. Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports. Nutrition. 2015;31:916–922. PubMed

Bloomer R.J. Effect of exercise on oxidative stress biomarkers. Adv Clin Chem. 2008;46:1–50. PubMed

Tyldum G.A., Schjerve I.E., Tjønna A.E., Kirkeby-Garstad I., Stolen T.O., Stølen T.O. Endothelian dysfunction induced by post-prandial lipemia; Complete protection afforded by high-intensity aerobic interval exercise. J Am Coll Cardiol. 2009;53:200–206. PubMed PMC

Parker L., McGuckin T.A., Leicht A.S. Influence of exercise intensity on systemic oxidative stress and antioxidant capacity. Clin Physiol Funct Imaging. 2014;34:377–382. PubMed

Clarkson P.M., Kearns A.K., Rouzier P., Rubin R., Thompson P.D. Serum creatine kinase levels and renal function measures in exertional muscle damage. Med Sci Sports Exerc. 2006;38:623–627. PubMed

Proske U., Morgan D.L. Muscle damage from eccentric exercise: mechanism, mechanical signs, adaptation and clinical applications. J Physiol. 2001;537:333–345. PubMed PMC

Kenney W.L., Wilmore J.H., Costill D.L. Human Kinetics; Champaign, IL: 2012. Physiology of sport and exercise.

Beaver W.L., Wassermann K., Whipp B.J. A new method for detecting anaerobic threshold by gas exchange. J Appl Physiol. 1986;60:2020–2027. PubMed

Kohn T.A., Essén-Gustavsson B., Myburgh K.H. Specific muscle adaptations in type II fibers after high-intensity interval training of well-trained runners. Scand J Med Sci Sports. 2011;21:765–772. PubMed

Borg G.A. Psychophysical bases of perceived exertion. Med Sci Sports Exerc. 1982;14:377–381. PubMed

Task Force of the European Society of Cardiology, North American Society of Pacing and Electrophysiology Heart rate variability: standards of measurement, physiological interpretation, and clinical use. Circulation. 1996;93:1043–1065. PubMed

Plews D.J., Laursen P.B., Stanley J., Kilding A.E., Buchheit M. Training adaptation and heart rate variability in elite endurance athletes: opening the door to effective monitoring. Sports Med. 2013;43:773–781. PubMed

Al Haddad H., Laursen P.B., Chollet D., Ahmaidi S., Buchheit M. Reliability of resting and postexercise heart rate measures. Int J Sports Med. 2011;32:598–605. PubMed

Penttilä J., Helminen A., Jartti T., Kuusela T., Huikuri H.V., Tulppo M.P. Time domain, geometrical and frequency domain analysis of cardiac vagal outflow: effects of various respiratory patterns. Clin Physiol. 2001;21:365–376. PubMed

Batterham A.M., Hopkins W.G. Making meaningful inferences about magnitudes. Int J Sports Physiol Perform. 2006;1:50–57. PubMed

Hopkins W.G. Spreadsheets for analysis of controlled trials, with adjustments for a subject characteristic. Sportscience. 2006;10:46–50.

Midgley A.W., McNaughton L.R. Time at or near VO2max during continuous and intermittent running. A review with special reference to considerations for the optimisation of training protocols to elicit the longest time at or near VO2max. J Sports Med Phys Fitness. 2006;46:1–14. PubMed

Hayes P.R., Quinn M.D. A mathematical model for quantifying training. Eur J Appl Physiol. 2009;106:839–847. PubMed

Buchheit M., Laursen P.B., Ahmaidi S. Parasympathetic reactivation after repeated sprint exercise. Am J Physiol Heart Circ Physiol. 2007;293:H133–41. PubMed

Stanley J., Peake J.M., Buchheit M. Cardiac parasympathetic reactivation following exercise: implication for training prescription. Sports Med. 2013;43:1259–1277. PubMed

Buchheit M., Papelier Y., Laursen P.B., Ahmaidi S. Noninvasive assessment of cardiac parasympathetic function: post-exercise heart rate recovery or heart rate variability? Am J Physiol Heart Circ Physiol. 2007;293:H8–10. PubMed

Dixon E.M., Kamath M.V., McCartney N., Fallen E.L. Neural regulation of heart rate variability in endurance athletes and sedentary controls. Cardiovasc Res. 1992;26:713–719. PubMed

Seiler S., Haugen O., Kuffel E. Autonomic recovery after exercise in trained athletes: intensity and duration effects. Med Sci Sports Exerc. 2007;39:1366–1373. PubMed

Reihmane D., Jurka A., Tretjakovs P., Dela F. Increase in IL-6, TNF-α, and MMP-9, but not sICAM-1, concentrations depends on exercise duration. Eur J Appl Physiol. 2013;113:851–858. PubMed

Reihmane D., Dela F. Interleukin-6: possible biological roles during exercise. Eur J Sport Sci. 2014;14:242–250. PubMed

Croft L., Bartlett J.D., MacLaren D.P., Reilly T., Evans L., Mattey D.L. High-intensity interval training attenuates the exercise-induced increase in plasma IL-6 in response to acute exercise. Appl Physiol Nutr Metab. 2009;34:1098–1107. PubMed

Yfanti C., Fischer C.P., Nielsen S., Akerström T., Nielsen A.R., Veskoukis A.S. Role of vitamin C and E supplementation on IL-6 in response to training. J Appl Physiol. 2012;112:990–1000. PubMed

Fischer C.P., Plomgaard P., Hansen A.K., Pilegaard H., Saltin B., Pedersen B.K. Endurance training reduces the contraction-induced interleukin-6 mRNA expression in human skeletal muscle. Am J Physiol Endocrinol Metab. 2004;287:E1189–94. PubMed

Fisher G., Schwartz D.D., Quindry J., Barberio M.D., Foster E.B., Jones K.W. Lymphocyte enzymatic antioxidant responses to oxidative stress following high-intensity interval exercise. J Appl Physiol. 2011;110:730–737. PubMed

Bogdanis G.C., Stavrinou P., Fatouros I.G., Philippou A., Chatzinikolaou A., Draganidis D. Short-term high-intensity interval exercise training attenuates oxidative stress responses and improves antioxidant status in healthy humans. Food Chem Toxicol. 2013;61:171–177. PubMed

Nosaka K., Newton M. Concentric or eccentric training effect on eccentric exercise-induced muscle damage. Med Sci Sports Exerc. 2002;34:63–69. PubMed

Chen T.C., Nosaka K., Sacco P. Intensity of eccentric exercise, shift of optimum angle, and the magnitude of repeated-bout effect. J Appl Physiol. 2007;102:992–999. PubMed

Spiering B.A., Kraemer W.J., Anderson J.M., Armstrong L.E., Nindl B.C., Volek J.S. Resistance exercise biology manipulation of resistance exercise programme variables determines the responses of cellular and molecular signalling pathways. Sports Med. 2008;38:527–540. PubMed

Flann K.L., LaStayo P.C., McClain D.A., Hazel M., Lindstedt S.L. Muscle damage and muscle remodelling: no pain, no gain? J Exp Biol. 2011;214:674–679. PubMed

Newham D.J., Jones D.A., Edwards R.H. Plasma creatine kinase changes after eccentric and concentric contractions. Muscle Nerve. 1986;9:59–63. PubMed

Acute and Post-Exercise Physiological Responses to High-Intensity Interval Training in Endurance and Sprint Athletes