A diversity of amoebae colonise the gills of farmed Atlantic salmon (Salmo salar) with amoebic gill disease (AGD)
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30447480
DOI
10.1016/j.ejop.2018.10.003
PII: S0932-4739(18)30087-7
Knihovny.cz E-zdroje
- Klíčová slova
- AGD, Amoebozoa, Aquaculture, Atlantic salmon, Discosea, Tubulinea,
- MeSH
- amébiáza parazitologie veterinární MeSH
- Amoebozoa klasifikace genetika ultrastruktura MeSH
- biodiverzita * MeSH
- fylogeneze MeSH
- mikroskopie MeSH
- nemoci ryb parazitologie MeSH
- respirační komplex IV genetika MeSH
- RNA ribozomální 18S genetika MeSH
- Salmo salar parazitologie MeSH
- transmisní elektronová mikroskopie MeSH
- žábry parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- respirační komplex IV MeSH
- RNA ribozomální 18S MeSH
Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim of the present study was to document the diversity of amoebae colonising the gills of AGD-affected farmed Atlantic salmon using a combination of morphological and sequence-based taxonomic methods. Amoebae were characterised morphologically via light microscopy and transmission electron microscopy, and by phylogenetic analyses based on the 18S rRNA gene and cytochrome oxidase subunit I (COI) gene. In addition to N. perurans, 11 other amoebozoans were isolated from the gills, and were classified within the genera Neoparamoeba, Paramoeba, Vexillifera, Pseudoparamoeba, Vannella and Nolandella. In some cases, such as Paramoeba eilhardi, this is the first time this species has been isolated from the gills of teleost fish. Furthermore, sequencing of both the 18S rRNA and COI gene revealed significant genetic variation within genera. We highlight that there is a far greater diversity of amoebae colonising AGD-affected gills than previously established.
Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic
The University of Queensland School of Biological Sciences Brisbane Queensland 4072 Australia
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