Nuclear hormone receptors: Ancient 9aaTAD and evolutionally gained NCoA activation pathways
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30468856
DOI
10.1016/j.jsbmb.2018.11.008
PII: S0960-0760(18)30465-5
Knihovny.cz E-resources
- Keywords
- 9aaTAD, CBP, HNF4, KIX, MED15, PPAR, RARa, VDL,
- MeSH
- Transcriptional Activation * MeSH
- Retinoic Acid Receptor alpha chemistry metabolism MeSH
- Caenorhabditis elegans chemistry metabolism MeSH
- Hepatocyte Nuclear Factor 4 chemistry metabolism MeSH
- Humans MeSH
- Models, Molecular MeSH
- Peptides chemistry metabolism MeSH
- PPAR gamma chemistry metabolism MeSH
- Protein Domains MeSH
- Caenorhabditis elegans Proteins chemistry metabolism MeSH
- Receptors, Cytoplasmic and Nuclear chemistry metabolism MeSH
- Receptors, Calcitriol chemistry metabolism MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Signal Transduction MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Retinoic Acid Receptor alpha MeSH
- Hepatocyte Nuclear Factor 4 MeSH
- NHR-49 protein, C elegans MeSH Browser
- Peptides MeSH
- PPAR gamma MeSH
- Caenorhabditis elegans Proteins MeSH
- RARA protein, human MeSH Browser
- Receptors, Cytoplasmic and Nuclear MeSH
- Receptors, Calcitriol MeSH
- VDR protein, human MeSH Browser
In higher metazoans, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor adaptors NCoA, which are absent in lower metazoans. The Nine amino acid TransActivation Domain, 9aaTAD, was reported for a large number of the transcription activators that recruit general mediators of transcription. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor of nematode C.elegans activates transcription as a small peptide. We showed that the ancient 9aaTAD domains are conserved in the nuclear hormone receptors including human HNF4, RARa, VDR and PPARg. Also their small 9aaTAD peptides effectively activated transcription in absence of the NCoA adaptors. We also showed that adjacent H11 domains in ancient and modern hormone receptors have an inhibitory effect on their 9aaTAD function.
References provided by Crossref.org
Myc 9aaTAD activation domain binds to mediator of transcription with superior high affinity
The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs
