FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30807658
DOI
10.1002/jcp.28394
Knihovny.cz E-resources
- Keywords
- DNA methylation, embryonic development, fertilization, fibroblast growth factors (FGFs), sperm-oocyte fusion, yak,
- MeSH
- Tetraspanin 28 genetics metabolism MeSH
- Tetraspanin 29 genetics metabolism MeSH
- Blastocyst drug effects physiology MeSH
- DNA (Cytosine-5-)-Methyltransferases genetics metabolism MeSH
- DNA (Cytosine-5-)-Methyltransferase 1 genetics metabolism MeSH
- DNA Methyltransferase 3B MeSH
- Embryonic Development drug effects genetics physiology MeSH
- Fertilization in Vitro veterinary MeSH
- Fertilization drug effects genetics physiology MeSH
- Fibroblast Growth Factor 10 administration & dosage physiology MeSH
- In Vitro Oocyte Maturation Techniques veterinary MeSH
- RNA, Messenger genetics metabolism MeSH
- Oocytes drug effects physiology MeSH
- Cattle embryology genetics physiology MeSH
- Pregnancy MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Cattle embryology genetics physiology MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Tetraspanin 28 MeSH
- Tetraspanin 29 MeSH
- DNA (Cytosine-5-)-Methyltransferases MeSH
- DNA (Cytosine-5-)-Methyltransferase 1 MeSH
- Fibroblast Growth Factor 10 MeSH
- RNA, Messenger MeSH
The fusion of sperm and oocytes determines the fertilization competence and subsequent development of embryos, which, in turn, can be affected by various proteins and DNA methylation. However, several factors in this whole regulation process remain unknown, especially in yaks. Here, we report that fibroblast growth factor 10 (FGF10) is an important growth factor that can enhance the maturation rate of yak oocytes and the motility of frozen spermatozoa. Subsequent blastocyst quality was also improved by increasing the total cell number and level of pregnancy-associated protein in blastocysts. These effects were significantly high in the group that received the 5 ng/ml FGF10 treatment, during both in vitro maturation (IVM) and capacitation. Our data show that the effects of FGF10 were dose-dependent at vital steps of embryogenesis in vitro. Furthermore, quantitative polymerase chain reaction, western blot analysis, and immunofluorescence demonstrated that the levels of CD9, CD81, DNMT1, and DNMT3B in both mature cumulus-oocyte complexes and capacitated sperms were regulated by FGF10, which was also highly expressed in the group treated with 5 ng/ml FGF10 during both IVM and capacitation. From our present study, we concluded that FGF10 promotes yak oocyte fertilization competence and subsequent blastocyst quality, and could also regulate CD9, CD81, DNMT1, and DNMT3B to optimize sperm-oocyte interactions and DNA methylation during fertilization.
College of Veterinary Medicine Gansu Agricultural University Lanzhou Gansu China
Department of Veterinary Medicine Sindh Agriculture University Tandojam Pakistan
References provided by Crossref.org
Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos
