Antibiotic susceptibility and production of endotoxin by Ochrobactrum anthropi isolated from environment and from patients with cystic fibrosis
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
PubMed
30919306
DOI
10.1007/s12223-019-00700-8
PII: 10.1007/s12223-019-00700-8
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Bacterial MeSH
- Cystic Fibrosis microbiology MeSH
- Child MeSH
- Endotoxins metabolism MeSH
- Gram-Negative Bacterial Infections microbiology MeSH
- Humans MeSH
- Limulus Test MeSH
- Microbial Sensitivity Tests MeSH
- Environmental Microbiology MeSH
- Adolescent MeSH
- Ochrobactrum anthropi drug effects isolation & purification metabolism MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Adolescent MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Endotoxins MeSH
The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).
Centre of Hyperbaric Medicine Ostrava City Hospital Ostrava Czech Republic
Department of Immunology and Alergology Public Health Institute Ostrava Ostrava Czech Republic
Department of Neonatology University Hospital Olomouc Olomouc Czech Republic
Faculty of Public Health Slovak Medical University in Bratislava Bratislava Slovak Republic
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