The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence MeSH
• cystická fibróza mikrobiologie   MeSH
• dítě MeSH
• endotoxiny metabolismus   MeSH
• gramnegativní bakteriální infekce mikrobiologie   MeSH
• lidé MeSH
• Limulus test MeSH
• mikrobiální testy citlivosti MeSH
• mikrobiologie životního prostředí MeSH
• mladiství MeSH
• Ochrobactrum anthropi účinky léků   izolace a purifikace   metabolismus   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

Similar to lipopolysaccharide (LPS), a product of Gram-negative bacteria, the signal macromolecules of Gram-positive bacteria lipoteichoic acid (LTA) and peptidoglycan (PGN) possess multiple biological activities. They may be a source of misinterpretation of experimental findings. We have found that not only LPS but also LTA and PGN can be detected by the Limulus amebocyte lysate (LAL) assay. All of them stimulate the high output in vitro nitric oxide (NO) production of in rat peritoneal cells. The onset of the NO enhancement was observed with 25-100pg/ml of LPS and 25-100ng/ml of PGN and LTA. Polymyxin B (PMX), if applied at concentration 10,000-fold higher than that of LPS, can completely inhibit the NO and LAL binding responses of LPS. The NO-stimulatory and LAL-binding properties of LTA and PGN are not eliminated by PMX. Handling of LPS contamination with PMX may be associated with serious problems because it possesses intrinsic biological activity and becomes cytotoxic at concentration >25μg/ml. The present findings suggest a convenient alternative avoiding these issues. As monitored by the NO and LAL assays, even high amounts of LPS as well as PGN and LTA can be removed by molecular mass cutoff microfiltration. All types of the filters (3kDa to 100kDa) are equally effective. It is suggested that the microfiltration procedure may be considered as a preferable, general and easy method of sample decontamination.

• MeSH
• Bacillus subtilis chemie   izolace a purifikace   MeSH
• buněčné linie MeSH
• centrifugace MeSH
• Escherichia coli chemie   izolace a purifikace   MeSH
• filtrace * MeSH
• krysa rodu rattus MeSH
• kyseliny teichoové analýza   MeSH
• Limulus test * MeSH
• lipopolysacharidy analýza   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• oxid dusnatý biosyntéza   metabolismus   MeSH
• peptidoglykan analýza   MeSH
• potkani Wistar MeSH
• viabilita buněk MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Stanovení hladin 1,3-beta-D-glukanu v klinickém materiálu patří do uznávaného spektra nekultivačních metod pro diagnostiku hlubokých houbových infekcí. Interpretace výsledků tohoto testu je mnohdy komplikovaná – nicméně ve spojení s pneumocystovou pneumonií má vyšetření řadu výhod a vhodně doplňuje standardní vyšetřovací metody, jako jsou mikroskopie a PCR.

The importance of 1,3-beta-D-glucan in diagnostics of pneumocystis pneumonia Determining the levels of 1,3-beta-D-glucan in the clinical material is a part of the spectrum of recognised non-cultivation methods for diagnosing deep, fungal infections. Interpretation of the results of this test is often complicated – however, it has many advantages in relation to pneumocystis pneumonia and it is a good addition to standard methods like microscopy and PCR.

• MeSH
• beta-glukany * diagnostické užití   krev   MeSH
• lidé MeSH
• Limulus test * metody   MeSH
• plicní mykózy diagnóza   mikrobiologie   MeSH
• pneumocystová pneumonie * diagnóza   mikrobiologie   MeSH
• senzitivita a specificita MeSH
• Check Tag
• lidé MeSH

• Klíčová slova
• heterodimer,
• MeSH
• beta-glukany MeSH
• bezobratlí * MeSH
• hemolymfa * MeSH
• lidé MeSH
• Limulus test MeSH
• lipopolysacharidy * MeSH
• přirozená imunita MeSH
• proteasy MeSH
• serinové proteasy * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• glukosamin analýza   MeSH
• LD50 MeSH
• Limulus test MeSH
• lipid A chemie   toxicita   MeSH
• mastné kyseliny analýza   MeSH
• myši MeSH
• sacharidy analýza   MeSH
• Shigella dysenteriae chemie   klasifikace   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH

• MeSH
• bakteriální infekce diagnóza   MeSH
• dítě MeSH
• lidé MeSH
• Limulus test MeSH
• nemoci novorozenců MeSH
• novorozenec MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• novorozenec MeSH

• MeSH
• endotoxiny analýza   MeSH
• Limulus test MeSH
• parenterální infuze MeSH
• pyrogeny analýza   MeSH

• Konspekt
• Lékařské vědy. Lékařství

• MeSH
• endotoxiny krev   MeSH
• lidé MeSH
• Limulus test metody   MeSH
• popálení diagnóza   komplikace   MeSH
• Check Tag
• lidé MeSH