Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor α, interleukin-1β, and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-κB phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass.

• MeSH
• CD11b Antigen metabolism   MeSH
• Antigens, CD metabolism   MeSH
• Cytokines blood   MeSH
• Eutrophication MeSH
• GPI-Linked Proteins metabolism   MeSH
• Cells, Cultured MeSH
• Laboratories MeSH
• Leukocytes, Mononuclear drug effects   metabolism   MeSH
• Humans MeSH
• Lipopolysaccharides toxicity   MeSH
• Microcystis * MeSH
• Cell Adhesion Molecules metabolism   MeSH
• Immunity, Innate drug effects   MeSH
• Pyrogens toxicity   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Zvýšená teplota či horečka patří mezi nejčastější příznaky zánětlivých akutních či chronických onemocnění. Nejde tudíž o nemoc, nýbrž příznak. Regulaci teploty zajišťuje „termostat" v mezimozku. Významnou roli hrají „pyrogeny" půobící jedndnak mimotělesně, jednak tvořené v organismu. Samotná horečka není nemocí, nýbž jejím projevem, což ovlivňuje i její léčbu. Léčebné postupy zahrnují jednak fyzikální metody (ochlazování), a jednak farmakoterapii aplikací antipyretik. Kauzální terapie se ovšem týká vyvolavatele základního onemocnění.

• MeSH
• Antipyretics pharmacology   therapeutic use   MeSH
• Child MeSH
• Fever * drug therapy   therapy   MeSH
• Hypothalamus physiology   MeSH
• Humans MeSH
• Pyrogens MeSH
• Check Tag
• Child MeSH
• Humans MeSH

• MeSH
• Antipyretics toxicity   MeSH
• Autoimmune Diseases diagnosis   immunology   MeSH
• Autoantibodies analysis   MeSH
• Diagnosis, Differential MeSH
• Fever * diagnosis   etiology   physiopathology   therapy   MeSH
• Communicable Diseases immunology   microbiology   physiopathology   MeSH
• Humans MeSH
• Paraneoplastic Syndromes MeSH
• Pyrogens classification   metabolism   adverse effects   MeSH
• Check Tag
• Humans MeSH

Horečka je častým příznakem kriticky nemocných na jednotkách intenzivní resuscitační péče. Představuje fyziologickou reakci na zevní i vnitřní podněty. Prostřednictvím dotazníkového šetření autoři v průzkumu hodnotili postupy sester při ošetřování pacienta s febrilií na intenzivních pracovištích tří ostravských nemocnic.

• Keywords
• endogenní pyrogeny, akutní zánětlivé procesy, intenzivní péče, ošetřovatelské intervence, fyzikální chlazení,
• MeSH
• Refrigeration utilization   MeSH
• Fever mortality   therapy   MeSH
• Infections complications   physiopathology   MeSH
• Humans MeSH
• Nursing Care MeSH
• Critical Care methods   MeSH
• Surveys and Questionnaires utilization   MeSH
• Pyrogens adverse effects   therapeutic use   MeSH
• Data Collection MeSH
• Check Tag
• Humans MeSH

• MeSH
• Child MeSH
• Fever etiology   drug therapy   physiopathology   MeSH
• Humans MeSH
• Analgesics, Non-Narcotic MeSH
• Pyrogens physiology   MeSH
• Body Temperature Regulation physiology   MeSH
• Check Tag
• Child MeSH
• Humans MeSH
• Publication type
• Congress MeSH

• MeSH
• Fever diagnosis   etiology   complications   therapy   MeSH
• Communicable Diseases therapy   MeSH
• Humans MeSH
• Drinking MeSH
• Pyrogens MeSH
• Thermometers MeSH
• Check Tag
• Humans MeSH

• MeSH
• Fever etiology   physiopathology   therapy   MeSH
• Pyrogens physiology   adverse effects   MeSH
• Body Temperature physiology   drug effects   MeSH

• MeSH
• Bacterial Toxins immunology   MeSH
• Borrelia Infections immunology   MeSH
• Leukocytes, Mononuclear physiology   drug effects   MeSH
• Humans MeSH
• Pyrogens immunology   toxicity   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Comparative Study MeSH

• MeSH
• Fever virology   MeSH
• Interleukins immunology   MeSH
• Humans MeSH
• Pyrogens immunology   MeSH
• Check Tag
• Humans MeSH

Dizertační práce, která se zaměřila na pyrogenní účinky cytokinů na hnědou tukovou tkáň a na buněčné mediátory a regulační vazby.

• MeSH
• Cell Line MeSH
• Cytokines MeSH
• Adipose Tissue, Brown MeSH
• Pyrogens MeSH
• Thermogenesis MeSH
• Publication type
• Academic Dissertation MeSH

• Conspectus
• Fyziologie člověka a srovnávací fyziologie
• NML Fields
• fyziologie
• biochemie