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Antibiotic susceptibility and production of endotoxin by Ochrobactrum anthropi isolated from environment and from patients with cystic fibrosis

D. Chmelař, O. Holý, I. Kasáková, M. Hájek, A. Lazarová, C. Gonzalez-Rey, J. Lasák, V. Raclavský, I. Čižnár,

. 2019 ; 64 (6) : 861-865. [pub] 20190327

Language English Country United States

Document type Comparative Study, Journal Article

Persistent link   https://www.medvik.cz/link/bmc20008026

The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).

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$a The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).
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$a Holý, Ondřej $u Department of Public Health, Faculty of Medicine and Dentistry, Palacký University Olomouc, Hněvotínská 3, 775 15, Olomouc, Czech Republic. Holy.ondrej@seznam.cz.
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$a Kasáková, Iveta $u Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic.
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$a Hájek, Michal $u Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic. Centre of Hyperbaric Medicine, Ostrava City Hospital, Ostrava, Czech Republic.
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$a Lazarová, Anna $u Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic. Department of Immunology and Alergology, Public Health Institute Ostrava, Ostrava, Czech Republic.
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$a Gonzalez-Rey, Carlos $u Swedish Orphan Biovitrum AB, Stockholm, Sweden.
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$a Lasák, Jakub $u Department of Neonatology, University Hospital Olomouc, Olomouc, Czech Republic.
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$a Raclavský, Vladislav $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic.
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$a Čižnár, Ivan $u Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic. Faculty of Public Health, Slovak Medical University in Bratislava, Bratislava, Slovak Republic.
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