Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30925271
DOI
10.1016/j.jpba.2019.03.039
PII: S0731-7085(18)32571-8
Knihovny.cz E-zdroje
- Klíčová slova
- HPLC-ESI-MS/MS, Kynurenine catabolic pathway, Latent toxoplasmosis, Principal component analysis,
- MeSH
- krevní plazma metabolismus MeSH
- krysa rodu Rattus MeSH
- kynurenin analogy a deriváty krev metabolismus MeSH
- kyselina chinolinová krev metabolismus MeSH
- kyselina hydroxyindoloctová krev metabolismus MeSH
- mozek metabolismus MeSH
- neurotransmiterové látky metabolismus MeSH
- potkani Wistar MeSH
- serotonin metabolismus MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- toxoplazmóza krev metabolismus MeSH
- tryptofan metabolismus MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 3-hydroxykynurenine MeSH Prohlížeč
- kynurenin MeSH
- kyselina chinolinová MeSH
- kyselina hydroxyindoloctová MeSH
- neurotransmiterové látky MeSH
- serotonin MeSH
- tryptofan MeSH
The aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biological matrices (brain tissue and blood plasma). The developed analytical method consists of analytes separation from the biological matrices by protein precipitation (blood plasma) or solvent extraction (brain tissue), derivatization of the analytes and their detection by high-performance liquid chromatography combined with mass spectrometry. Individual steps of the whole process were optimized and the method was validated in the terms of selectivity, linearity (R2≥0.980), precision (RSD ≤ 13.3%), recovery (≥82.0%), limit of detection (1.8 ng/mL of blood plasma, 2.2 pg/mg of brain tissue) and limit of quantification (2.5 ng/mL of blood plasma, 2.8 pg/mg of brain tissue). The method was subsequently verified by an animal study, where the concentration levels of the analytes in biological matrices (blood plasma and brain tissue) of T. gondii - infected rats and control animals were compared. All the data obtained from the animal study were statistically evaluated. Increased concentration levels of kynurenine catabolic pathway metabolites (e.g. kynurenine, 3-hydroxykynurenine, quinolinic acid) were observed in the case of T. gondii - infected rats in contrast to the control group. The opposite effect was determined in the case of serotonin and its metabolite 5-hydroxyindoleacetic acid, where higher concentration levels were found in blood plasma of healthy subjects. Finally, Principal Component Analysis (PCA) was utilized for a score plot formation. PCA score plots have demonstrated the similarities of individuals within each group and the differences among the groups.
3rd Faculty of Medicine Charles University Ruská 87 100 00 Prague 10 Czech Republic
National Institute of Mental Health Topolová 748 250 67 Klecany Czech Republic
The National Institute of Public Health Šrobárova 48 100 42 Prague 10 Czech Republic
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