The aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biological matrices (brain tissue and blood plasma). The developed analytical method consists of analytes separation from the biological matrices by protein precipitation (blood plasma) or solvent extraction (brain tissue), derivatization of the analytes and their detection by high-performance liquid chromatography combined with mass spectrometry. Individual steps of the whole process were optimized and the method was validated in the terms of selectivity, linearity (R2≥0.980), precision (RSD ≤ 13.3%), recovery (≥82.0%), limit of detection (1.8 ng/mL of blood plasma, 2.2 pg/mg of brain tissue) and limit of quantification (2.5 ng/mL of blood plasma, 2.8 pg/mg of brain tissue). The method was subsequently verified by an animal study, where the concentration levels of the analytes in biological matrices (blood plasma and brain tissue) of T. gondii - infected rats and control animals were compared. All the data obtained from the animal study were statistically evaluated. Increased concentration levels of kynurenine catabolic pathway metabolites (e.g. kynurenine, 3-hydroxykynurenine, quinolinic acid) were observed in the case of T. gondii - infected rats in contrast to the control group. The opposite effect was determined in the case of serotonin and its metabolite 5-hydroxyindoleacetic acid, where higher concentration levels were found in blood plasma of healthy subjects. Finally, Principal Component Analysis (PCA) was utilized for a score plot formation. PCA score plots have demonstrated the similarities of individuals within each group and the differences among the groups.
- MeSH
- krevní plazma metabolismus MeSH
- krysa rodu rattus MeSH
- kynurenin analogy a deriváty krev metabolismus MeSH
- kyselina chinolinová krev metabolismus MeSH
- kyselina hydroxyindoloctová krev metabolismus MeSH
- mozek metabolismus MeSH
- neurotransmiterové látky metabolismus MeSH
- potkani Wistar MeSH
- serotonin metabolismus MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- toxoplazmóza krev metabolismus MeSH
- tryptofan metabolismus MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Je popsána kazuistika nemocného se syndromem karcinoidu, který se manifestoval postižením pravostran-ných srdečních oddílů, obstrukcí dýchacích cest a červenofialovým zabarvením horní poloviny těla. Diagnózu správně stanovil kardiolog při transezofageálním vyšetření. Po intenzivní léčbě chirurgické a medi-kamentózní červenofialové zabarvení pacienta prakticky zmizelo.
In this čase, a patient with carcinoid syndrome has been described with manifestation of right heart failure, pulmonary obstruction and red-blue skin colour of upper part of the body. The diagnosis has been correct- ly established after trans-oesophageal echographia examination by cardiologist. Following an intensive sur- gical and drug treatment, the skin colour has improved with disappearance of the red-blue colour.
- MeSH
- barva MeSH
- echokardiografie transezofageální MeSH
- karcinoid diagnóza etiologie chirurgie MeSH
- kyselina hydroxyindoloctová krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- somatostatin analogy a deriváty aplikace a dávkování škodlivé účinky MeSH
- syndrom MeSH
- trikuspidální chlopeň fyziologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- přehledy MeSH
- MeSH
- aktivace trombocytů fyziologie MeSH
- beta-thromboglobulin metabolismus MeSH
- dospělí MeSH
- hypertenze krev patofyziologie MeSH
- kyselina hydroxyindoloctová krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- serotonin krev MeSH
- stárnutí metabolismus MeSH
- trombocyty metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
