• Je něco špatně v tomto záznamu ?

Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis

J. Vondroušová, M. Mikoška, K. Syslová, A. Böhmová, H. Tejkalová, L. Vacek, P. Kodym, D. Krsek, J. Horáček,

. 2019 ; 170 (-) : 139-152. [pub] 20190318

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19034684

The aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biological matrices (brain tissue and blood plasma). The developed analytical method consists of analytes separation from the biological matrices by protein precipitation (blood plasma) or solvent extraction (brain tissue), derivatization of the analytes and their detection by high-performance liquid chromatography combined with mass spectrometry. Individual steps of the whole process were optimized and the method was validated in the terms of selectivity, linearity (R2≥0.980), precision (RSD ≤ 13.3%), recovery (≥82.0%), limit of detection (1.8 ng/mL of blood plasma, 2.2 pg/mg of brain tissue) and limit of quantification (2.5 ng/mL of blood plasma, 2.8 pg/mg of brain tissue). The method was subsequently verified by an animal study, where the concentration levels of the analytes in biological matrices (blood plasma and brain tissue) of T. gondii - infected rats and control animals were compared. All the data obtained from the animal study were statistically evaluated. Increased concentration levels of kynurenine catabolic pathway metabolites (e.g. kynurenine, 3-hydroxykynurenine, quinolinic acid) were observed in the case of T. gondii - infected rats in contrast to the control group. The opposite effect was determined in the case of serotonin and its metabolite 5-hydroxyindoleacetic acid, where higher concentration levels were found in blood plasma of healthy subjects. Finally, Principal Component Analysis (PCA) was utilized for a score plot formation. PCA score plots have demonstrated the similarities of individuals within each group and the differences among the groups.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19034684
003      
CZ-PrNML
005      
20191015094904.0
007      
ta
008      
191007s2019 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.jpba.2019.03.039 $2 doi
035    __
$a (PubMed)30925271
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Vondroušová, Jana $u Department of Organic Technology, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
245    10
$a Monitoring of kynurenine pathway metabolites, neurotransmitters and their metabolites in blood plasma and brain tissue of individuals with latent toxoplasmosis / $c J. Vondroušová, M. Mikoška, K. Syslová, A. Böhmová, H. Tejkalová, L. Vacek, P. Kodym, D. Krsek, J. Horáček,
520    9_
$a The aim of the presented work was to develop a highly sensitive, accurate and rapid analytical method for the determination of concentration levels of tryptophan and its metabolites of kynurenine catabolic pathway, as well as neurotransmitters and their metabolites in complex biological matrices (brain tissue and blood plasma). The developed analytical method consists of analytes separation from the biological matrices by protein precipitation (blood plasma) or solvent extraction (brain tissue), derivatization of the analytes and their detection by high-performance liquid chromatography combined with mass spectrometry. Individual steps of the whole process were optimized and the method was validated in the terms of selectivity, linearity (R2≥0.980), precision (RSD ≤ 13.3%), recovery (≥82.0%), limit of detection (1.8 ng/mL of blood plasma, 2.2 pg/mg of brain tissue) and limit of quantification (2.5 ng/mL of blood plasma, 2.8 pg/mg of brain tissue). The method was subsequently verified by an animal study, where the concentration levels of the analytes in biological matrices (blood plasma and brain tissue) of T. gondii - infected rats and control animals were compared. All the data obtained from the animal study were statistically evaluated. Increased concentration levels of kynurenine catabolic pathway metabolites (e.g. kynurenine, 3-hydroxykynurenine, quinolinic acid) were observed in the case of T. gondii - infected rats in contrast to the control group. The opposite effect was determined in the case of serotonin and its metabolite 5-hydroxyindoleacetic acid, where higher concentration levels were found in blood plasma of healthy subjects. Finally, Principal Component Analysis (PCA) was utilized for a score plot formation. PCA score plots have demonstrated the similarities of individuals within each group and the differences among the groups.
650    _2
$a zvířata $7 D000818
650    _2
$a mozek $x metabolismus $7 D001921
650    _2
$a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
650    _2
$a kyselina hydroxyindoloctová $x krev $x metabolismus $7 D006897
650    _2
$a kynurenin $x analogy a deriváty $x krev $x metabolismus $7 D007737
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a neurotransmiterové látky $x metabolismus $7 D018377
650    _2
$a krevní plazma $x metabolismus $7 D010949
650    _2
$a kyselina chinolinová $x krev $x metabolismus $7 D017378
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani Wistar $7 D017208
650    _2
$a serotonin $x metabolismus $7 D012701
650    _2
$a tandemová hmotnostní spektrometrie $x metody $7 D053719
650    _2
$a toxoplazmóza $x krev $x metabolismus $7 D014123
650    _2
$a tryptofan $x metabolismus $7 D014364
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mikoška, Miloš $u Department of Organic Technology, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
700    1_
$a Syslová, Kamila $u Department of Organic Technology, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic. Electronic address: syslovak@vscht.cz.
700    1_
$a Böhmová, Adéla $u Department of Organic Technology, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
700    1_
$a Tejkalová, Hana $u National Institute of Mental Health, Topolová 748, 250 67, Klecany, Czech Republic.
700    1_
$a Vacek, Lukáš $u Third Faculty of Medicine, Charles University, Ruská 87, 100 00, Prague 10, Czech Republic.
700    1_
$a Kodym, Petr $u The National Institute of Public Health, Šrobárova 48, 100 42, Prague 10, Czech Republic.
700    1_
$a Krsek, Daniel $u The National Institute of Public Health, Šrobárova 48, 100 42, Prague 10, Czech Republic.
700    1_
$a Horáček, Jiří $u National Institute of Mental Health, Topolová 748, 250 67, Klecany, Czech Republic.
773    0_
$w MED00002894 $t Journal of pharmaceutical and biomedical analysis $x 1873-264X $g Roč. 170, č. - (2019), s. 139-152
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30925271 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191015095329 $b ABA008
999    __
$a ok $b bmc $g 1451344 $s 1073234
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 170 $c - $d 139-152 $e 20190318 $i 1873-264X $m Journal of pharmaceutical and biomedical analysis $n J Pharm Biomed Anal $x MED00002894
LZP    __
$a Pubmed-20191007

Najít záznam

Citační ukazatele

Možnosti archivace