Different gut microbiome composition in obese Guizhou minipigs between female and castrated male
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
Grant support
[Qian Ke Zhong Yin Di [2016] 4007]
Central special fund for local science and technology development
[Qian Jiao He KY No. [2016] 182]
Project for growth of youth researcher of Education department of Guizhou province
PubMed
31011890
DOI
10.1007/s12223-019-00704-4
PII: 10.1007/s12223-019-00704-4
Knihovny.cz E-resources
- MeSH
- Bacteria classification genetics isolation & purification MeSH
- DNA, Bacterial genetics MeSH
- Feces microbiology MeSH
- Phylogeny MeSH
- Castration * MeSH
- Swine, Miniature MeSH
- Disease Models, Animal MeSH
- Obesity microbiology MeSH
- Swine MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Gastrointestinal Microbiome * genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
- RNA, Ribosomal, 16S MeSH
Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbiome composition is observed between the sexes. Here, we studied whether castrated male Guizhou minipigs with obesity own the same gut microbiome composition and microbial function predictions with those in obese females. We sequenced the hypervariable regions V3 to V4 of bacterial 16s rRNA of fecal samples collected from our study subjects. We observed that the operational taxonomic units were small, which suggested that the abundance of gut microbiome may be influenced by low genetic diversity of host. Our results also suggested that the castrated male has different gut microbial composition compared to the obese female. An increasing Firmicutes/Bacteroidetes ratio was observed in both castrated male and obese female groups, which suggested that the main adipogenic gut microorganism in obese Guizhou minipigs in our studies is the same with that in other obese mammals. However, we also observed that there were function prediction differences of obese Guizhou minipigs between female and castrated male, which suggested that the influence of gut microbiome on obesity between them is different.
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