A pilot study of the UVA-photoprotective potential of dehydrosilybin, isosilybin, silychristin, and silydianin on human dermal fibroblasts
Language English Media print-electronic
Document type Journal Article
Grant support
GACR 15-10897S
Grantová Agentura České Republiky
IGA_LF_2019_015
Univerzita Palackého v Olomouci (CZ)
IGA_LF_2018_012
Univerzita Palackého v Olomouci
RVO 61989592
Univerzita Palackého v Olomouci
PubMed
31079190
DOI
10.1007/s00403-019-01928-7
PII: 10.1007/s00403-019-01928-7
Knihovny.cz E-resources
- Keywords
- Cell culture, Flavonolignan, Heat shock protein, Metalloproteinase-1, Oxidative damage, UVA,
- MeSH
- Cytoprotection drug effects MeSH
- Fibroblasts drug effects radiation effects MeSH
- Glutathione metabolism MeSH
- Heme Oxygenase-1 metabolism MeSH
- DNA Breaks, Single-Stranded radiation effects MeSH
- Protein Carbonylation radiation effects MeSH
- Caspase 3 metabolism MeSH
- Cells, Cultured MeSH
- Skin radiation effects MeSH
- Humans MeSH
- Matrix Metalloproteinase 1 metabolism MeSH
- Sunscreening Agents pharmacology MeSH
- HSP70 Heat-Shock Proteins metabolism MeSH
- Reactive Oxygen Species metabolism MeSH
- Silymarin analogs & derivatives pharmacology MeSH
- Ultraviolet Rays adverse effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- dehydrosilybin MeSH Browser
- Glutathione MeSH
- Heme Oxygenase-1 MeSH
- HMOX1 protein, human MeSH Browser
- isosilybin MeSH Browser
- Caspase 3 MeSH
- Matrix Metalloproteinase 1 MeSH
- MMP1 protein, human MeSH Browser
- Sunscreening Agents MeSH
- HSP70 Heat-Shock Proteins MeSH
- Reactive Oxygen Species MeSH
- silidianin MeSH Browser
- silychristin MeSH Browser
- Silymarin MeSH
The exposure of naked unprotected skin to solar radiation may result in numerous acute and chronic undesirable effects. Evidence suggests that silymarin, a standardized extract from Silybum marianum (L.) Gaertn. seeds, and its major component silybin suppress UVB-induced skin damage. Here, we aimed to investigate the UVA-protective effects of silymarin's less abundant flavonolignans, specifically isosilybin (ISB), silychristin (SC), silydianin (SD), and 2,3-dehydrosilybin (DHSB). Normal human dermal fibroblasts (NHDF) pre-treated for 1 h with flavonolignans were then exposed to UVA light using a solar simulator. Their effects on reactive oxygen species (ROS), carbonylated proteins and glutathione (GSH) level, caspase-3 activity, single-strand breaks' (SSBs) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) were evaluated. The most pronounced preventative potential was found for DHSB, a minor component of silymarin, and SC, the second most abundant flavonolignan in silymarin. They had significant effects on most of the studied parameters. Meanwhile, a photoprotective effect of SC was mostly found at double the concentration of DHSB. ISB and SD protected against GSH depletion, the generation of ROS, carbonylated proteins and SSBs, and caspase-3 activation, but had no significant effect on MMP-1, HO-1, or HSP70. In summary, DHSB and to a lesser extent other silymarin flavonolignans are potent UVA-protective compounds. However, due to the in vitro phototoxic potential of DHSB published elsewhere, further studies are needed to exclude phototoxicity for humans as well as to confirm our results on human skin ex vivo and in vivo.
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