Genetic and chemical diversity among yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions based on iPBS markers and metabolomic fingerprinting
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31174035
DOI
10.1016/j.plaphy.2019.05.020
PII: S0981-9428(19)30208-6
Knihovny.cz E-zdroje
- Klíčová slova
- Genetic diversity, Metabolomic fingerprints, Retrotransposons, Smallanthus sonchifolius, iPBS,
- MeSH
- antioxidancia chemie MeSH
- Asteraceae chemie genetika MeSH
- diterpeny chemie MeSH
- genetická variace MeSH
- glykosylace MeSH
- hmotnostní spektrometrie MeSH
- kořeny rostlin chemie MeSH
- laktony chemie MeSH
- metabolomika MeSH
- multivariační analýza MeSH
- peptidové mapování MeSH
- retroelementy MeSH
- rostlinné extrakty chemie MeSH
- seskviterpeny chemie MeSH
- shluková analýza MeSH
- zeměpis MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Bolívie MeSH
- Česká republika MeSH
- Ekvádor MeSH
- Peru MeSH
- Názvy látek
- antioxidancia MeSH
- diterpeny MeSH
- laktony MeSH
- retroelementy MeSH
- rostlinné extrakty MeSH
- seskviterpeny MeSH
The present study is focused on the characterization of yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions from different geographic origins (Bolivia, Ecuador, and Peru) by iPBS markers and metabolomic fingerprinting. The results showed that the number of amplified polymorphic fragment levels ranged from 20 up to 27 with a level of polymorphism ranging from 80 to 100%. Five of the iPBS primers used in this study provided no specific banding pattern able to discriminate between the different yacon accessions. However, two iPBS primer pairs were able to separate Peru accessions from those of Ecuador and Bolivia. The UPLC-HRMS/MS-based metabolomic fingerprinting showed highly similar metabolomic fingerprints characterized by the accumulation of high quantities of sesquiterpene lactones and diterpenes, but no apparent geographic clustering. The present study demonstrates that yacon accessions from different geographical origins maintained ex situ (in the Czech Republic) present a rather low chemical and genetic diversity.
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