The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31176685
DOI
10.1016/j.abb.2019.06.001
PII: S0003-9861(18)30381-3
Knihovny.cz E-resources
- Keywords
- Embryonic stem cell, NADPH oxidase, Reactive oxygen species, p38 kinase,
- MeSH
- Cell Differentiation physiology MeSH
- Gene Knockdown Techniques MeSH
- Gene Knockout Techniques MeSH
- Cells, Cultured MeSH
- Membrane Potential, Mitochondrial physiology MeSH
- Mitochondria metabolism MeSH
- Mitogen-Activated Protein Kinase 14 genetics metabolism MeSH
- Mouse Embryonic Stem Cells metabolism MeSH
- Mice MeSH
- NADPH Oxidase 2 genetics metabolism MeSH
- Superoxides metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cybb protein, mouse MeSH Browser
- Mitogen-Activated Protein Kinase 14 MeSH
- NADPH Oxidase 2 MeSH
- Superoxides MeSH
P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
References provided by Crossref.org
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