Hypersaline aquatic and terrestrial ecosystems display a cosmopolitan distribution. These environments teem with microbes and harbor a plethora of prokaryotic lineages that evaded ecological characterization due to the prior inability to cultivate them or to access their genomic information. In order to close the current knowledge gap, we performed two sampling and isolation campaigns in the saline soils of the Odiel Saltmarshes and the salterns of Isla Cristina (Huelva, Spain). From the isolated haloarchaeal strains subjected to high-throughput phylogenetic screening, two were chosen (F15BT and F9-27T) for physiological and genomic characterization due of their relatedness to the genus Halonotius. Comparative genomic analyses were carried out between the isolated strains and the genomes of previously described species Halonotius pteroides CECT 7525T, Halonotius aquaticus F13-13T and environmentaly recovered metagenome-assembled representatives of the genus Halonotius. The topology of the phylogenomic tree showed agreement with the phylogenetic ones based on 16S rRNA and rpoB' genes, and together with average amino acid and nucleotide identities suggested the two strains as novel species within the genus. We propose the names Halonotius terrestris sp. nov. (type strain F15BT = CECT 9688T = CCM 8954T) and Halonotius roseus sp. nov. (type strain F9-27T = CECT 9745T = CCM 8956T) for these strains. Comparative genomic analyses within the genus highlighted a typical salt-in signature, characterized by acidic proteomes with low isoelectric points, and indicated heterotrophic aerobic lifestyles. Genome-scale metabolic reconstructions revealed that the newly proposed species encode all the necessary enzymatic reactions involved in cobalamin (vitamin B12) biosynthesis. Based on the worldwide distribution of the genus and its abundance in hypersaline habitats we postulate that its members perform a critical function by being able to provide "expensive" commodities (i.e., vitamin B12) to the halophilic microbial communities at large.

Department of Aquatic Microbial Ecology Institute of Hydrobiology Biology Centre of the Academy of Sciences of the Czech Republic České Budějovice Czechia

Department of Microbiology and Parasitology Faculty of Pharmacy University of Seville Seville Spain

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Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215 403–410. 10.1016/S0022-2836(05)80360-2 PubMed DOI

Amoozegar M. A., Siroosi M., Atashgahi S., Smidt H., Ventosa A. (2017). Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes. Microbiology 163 623–645. 10.1099/mic.0.000463 PubMed DOI

Anderson I., Scheuner C., Göker M., Mavromatis K., Hooper S. D., Porat I., et al. (2011). Novel insights into the diversity of catabolic metabolism from ten haloarchaeal genomes. PLoS One 6:e20237. 10.1371/journal.pone.0020237 PubMed DOI PMC

Arahal D. R., Dewhirst F. E., Paster B. J., Volcani B. E., Ventosa A. (1996). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl. Environ. Microbiol. 62 3779–3786. PubMed PMC

Auch A. F., Klenk H.-P., Göker M. (2010). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand. Genomic Sci. 2 142–148. 10.4056/sigs.541628 PubMed DOI PMC

Aziz R. K., Bartels D., Best A. A., DeJongh M., Disz T., Edwards R. A., et al. (2008). The RAST server: rapid annotations using subsystems technology. BMC Genomics 9:75. 10.1186/1471-2164-9-75 PubMed DOI PMC

Bankevich A., Nurk S., Antipov D., Gurevich A. A., Dvorkin M., Kulikov A. S., et al. (2012). SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19 455–477. 10.1089/cmb.2012.0021 PubMed DOI PMC

Becker E. A., Seitzer P. M., Tritt A., Larsen D., Krusor M., Yao A. I., et al. (2014). Phylogenetically driven sequencing of extremely halophilic archaea reveals strategies for static and dynamic osmo-response. PLoS Genet. 10:e1004784. 10.1371/journal.pgen.1004784 PubMed DOI PMC

Bertrand E. M., Saito M. A., Jeon Y. J., Neilan B. A. (2011). Vitamin B12 biosynthesis gene diversity in the Ross Sea: the identification of a new group of putative polar B12 biosynthesizers. Environ. Microbiol. 13 1285–1298. 10.1111/j.1462-2920.2011.02428.x PubMed DOI

Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., et al. (2006). The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity. BMC Genomics 7:169. 10.1186/1471-2164-7-169 PubMed DOI PMC

Boucher Y., Douady C. J., Sharma A. K., Kamekura M., Doolittle W. F. (2004). Intragenomic heterogeneity and intergenomic recombination among haloarchaeal rRNA genes. J. Bacteriol. 186 3980–3990. 10.1128/JB.186.12.3980-3990.2004 PubMed DOI PMC

Burns D. G., Camakaris H. M., Janssen P. H., Dyall-Smith M. L. (2004). Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. Appl. Environ. Microbiol. 70 5258–5265. 10.1128/AEM.70.9.5258-5265.2004 PubMed DOI PMC

Burns D. G., Janssen P. H., Itoh T., Kamekura M., Echigo A., Dyall-Smith M. L. (2010). Halonotius pteroides gen. nov., sp. nov., an extremely halophilic archaeon recovered from a saltern crystallizer. Int. J. Syst. Evol. Microbiol. 60 1196–1199. 10.1099/ijs.0.010017-0 PubMed DOI

Bushnell B. (2016). BBMap Project. Available at: http://sourceforge.net/projects/bbmap (accessed October, 2018).

Çınar S., Mutlu M. B. (2016). Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey). Extremophiles 20 589–601. 10.1007/s00792-016-0845-7 PubMed DOI

Corcelli A., Lobasso S. (2006). 25 characterization of lipids of Halophilic Archaea. Methods Microbiol. 35 585–613. 10.1016/S0580-9517(08)70028-X DOI

Criscuolo A., Gribaldo S. (2010). BMGE (Block Mapping and Gathering with Entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments. BMC Evol. Biol. 10:210. 10.1186/1471-2148-10-210 PubMed DOI PMC

DeLong E. F. (1992). Archaea in coastal marine environments. Proc. Natl. Acad. Sci. U.S.A. 89 5685–5689. 10.1073/PNAS.89.12.5685 PubMed DOI PMC

Deole R., Challacombe J., Raiford D. W., Hoff W. D. (2013). An extremely halophilic proteobacterium combines a highly acidic proteome with a low cytoplasmic potassium content. J. Biol. Chem. 288 581–588. 10.1074/jbc.M112.420505 PubMed DOI PMC

Doxey A. C., Kurtz D. A., Lynch M. D., Sauder L. A., Neufeld J. D. (2015). Aquatic metagenomes implicate Thaumarchaeota in global cobalamin production. ISME J. 9 461–471. 10.1038/ismej.2014.142 PubMed DOI PMC

Durán-Viseras A., Ventosa A., Sánchez-Porro C. (2019). Halonotius aquaticus sp. nov., a new haloarchaeon isolated from a marine saltern. Int. J. Syst. Evol. Microbiol. 69 1306–1312. 10.1099/ijsem.0.003309 PubMed DOI

Eddy S. R. (2011). Accelerated profile HMM searches. PLoS Comput. Biol. 7:e1002195. 10.1371/journal.pcbi.1002195 PubMed DOI PMC

Edgar R. C. (2010). Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26 2460–2461. 10.1093/bioinformatics/btq461 PubMed DOI

Empadinhas N., Da Costa M. S. (2008). Osmoadaptation mechanisms in prokaryotes: distribution of compatible solutes. Int. Microbiol. 11 151–161. 10.2436/20.1501.01.55 PubMed DOI

Falb M., Müller K., Königsmaier L., Oberwinkler T., Horn P., von Gronau S., et al. (2008). Metabolism of halophilic archaea. Extremophiles 12 177–196. 10.1007/s00792-008-0138-x PubMed DOI PMC

Fernández A. B., Ghai R., Martín-Cuadrado A.-B., Sánchez-Porro C., Rodríguez-Valera F., Ventosa A. (2014a). Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline habitat assessed by metagenomics. FEMS Microbiol. Ecol. 88 623–635. 10.1111/1574-6941.12329 PubMed DOI

Fernández A. B., Vera-Gargallo B., Sánchez-Porro C., Ghai R., Papke R. T., Rodríguez-Valera F., et al. (2014b). Comparison of prokaryotic community structure from Mediterranean and Atlantic saltern concentrator ponds by a metagenomic approach. Front. Microbiol. 5:196. 10.3389/fmicb.2014.00196 PubMed DOI PMC

Finn R. D., Clements J., Arndt W., Miller B. L., Wheeler T. J., Schreiber F., et al. (2015). HMMER web server: 2015 update. Nucleic Acids Res. 43 W30–W38. 10.1093/nar/gkv397 PubMed DOI PMC

Frigaard N.-U., Martinez A., Mincer T. J., DeLong E. F. (2006). Proteorhodopsin lateral gene transfer between marine planktonic bacteria and Archaea. Nature 439 847–850. 10.1038/nature04435 PubMed DOI

Fullmer M. S., Soucy S. M., Swithers K. S., Makkay A. M., Wheeler R., Ventosa A., et al. (2014). Population and genomic analysis of the genus Halorubrum. Front. Microbiol. 5:140. 10.3389/fmicb.2014.00140 PubMed DOI PMC

Galinski E. A., Trüper H. G. (1994). Microbial behaviour in salt-stressed ecosystems. FEMS Microbiol. Rev. 15 95–108. 10.1111/j.1574-6976.1994.tb00128.x DOI

Ghai R., Pašić L., Fernández A. B., Martin-Cuadrado A.-B., Mizuno C. M., McMahon K. D., et al. (2011). New abundant microbial groups in aquatic hypersaline environments. Sci. Rep. 1:135. 10.1038/srep00135 PubMed DOI PMC

Govorunova E. G., Sineshchekov O. A., Li H., Spudich J. L. (2017). Microbial rhodopsins: diversity, mechanisms, and optogenetic applications. Annu. Rev. Biochem. 86 845–872. 10.1146/annurev-biochem-101910-144233 PubMed DOI PMC

Gunde-Cimerman N., Plemenitaš A., Oren A. (2018). Strategies of adaptation of microorganisms of the three domains of life to high salt concentrations. FEMS Microbiol. Rev. 42 353–375. 10.1093/femsre/fuy009.Review PubMed DOI

Gurevich A., Saveliev V., Vyahhi N., Tesler G. (2013). QUAST: quality assessment tool for genome assemblies. Bioinformatics 29 1072–1075. 10.1093/bioinformatics/btt086 PubMed DOI PMC

Han R., Zhang X., Liu J., Long Q., Chen L., Liu D., et al. (2017). Microbial community structure and diversity within hypersaline Keke Salt Lake environments. Can. J. Microbiol. 63 895–908. 10.1139/cjm-2016-0773 PubMed DOI

Henriet O., Fourmentin J., Delincé B., Mahillon J. (2014). Exploring the diversity of extremely halophilic archaea in food-grade salts. Int. J. Food Microbiol. 191 36–44. 10.1016/J.IJFOODMICRO.2014.08.019 PubMed DOI

Hyatt D., LoCascio P. F., Hauser L. J., Uberbacher E. C. (2012). Gene and translation initiation site prediction in metagenomic sequences. Bioinformatics 28 2223–2230. 10.1093/bioinformatics/bts429 PubMed DOI

Kall L., Krogh A., Sonnhammer E. L. L. (2007). Advantages of combined transmembrane topology and signal peptide prediction–the Phobius web server. Nucleic Acids Res. 35 W429–W432. 10.1093/nar/gkm256 PubMed DOI PMC

Kalyaanamoorthy S., Minh B. Q., Wong T. K. F., von Haeseler A., Jermiin L. S. (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nat. Methods 14 587–589. 10.1038/nmeth.4285 PubMed DOI PMC

Katoh K., Standley D. M. (2013). MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30 772–780. 10.1093/molbev/mst010 PubMed DOI PMC

Klappenbach J. A., Goris J., Vandamme P., Coenye T., Konstantinidis K. T., Tiedje J. M. (2007). DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int. J. Syst. Evol. Microbiol. 57 81–91. 10.1099/ijs.0.64483-0 PubMed DOI

Konstantinidis K. T., Rosselló-Móra R., Amann R. (2017). Uncultivated microbes in need of their own taxonomy. ISME J. 11 2399–2406. 10.1038/ismej.2017.113 PubMed DOI PMC

Konstantinidis K. T., Tiedje J. M. (2004). Trends between gene content and genome size in prokaryotic species with larger genomes. Proc. Natl. Acad. Sci. U.S.A. 101 3160–3165. 10.1073/pnas.0308653100 PubMed DOI PMC

Konstantinidis K. T., Tiedje J. M. (2005a). Genomic insights that advance the species definition for prokaryotes. Proc. Natl. Acad. Sci. U.S.A. 102 2567–2572. 10.1073/pnas.0409727102 PubMed DOI PMC

Konstantinidis K. T., Tiedje J. M. (2005b). Towards a genome-based taxonomy for prokaryotes. J. Bacteriol. 187 6258–6264. 10.1128/JB.187.18.6258-6264.2005 PubMed DOI PMC

Kushwaha S. C., Juez-Perez G., Rodriguez-Valera F., Kates M., Kushner D. J. (1982). Survey of lipids of a new group of extremely of halophilic bacteria from salt ponds in Spain. Can. J. Microbiol. 28 1365–1372. 10.1139/m82-203 DOI

Le S. Q., Gascuel O. (2008). An improved general amino acid replacement matrix. Mol. Biol. Evol. 25 1307–1320. 10.1093/molbev/msn067 PubMed DOI

Li D., Liu C. M., Luo R., Sadakane K., Lam T. W. (2015). MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics 31 1674–1676. 10.1093/bioinformatics/btv033 PubMed DOI

López-Pérez M., Ghai R., Leon M. J., Rodríguez-Olmos Á, Copa-Patiño J. L., Soliveri J., et al. (2013). Genomes of “Spiribacter”, a streamlined, successful halophilic bacterium. BMC Genomics 14:787. 10.1186/1471-2164-14-787 PubMed DOI PMC

Löytynoja A. (2014). Phylogeny-aware alignment with PRANK. Methods Mol. Biol. 1079 155–170. 10.1007/978-1-62703-646-7_10 PubMed DOI

Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, et al. (2004). ARB: a software environment for sequence data. Nucleic Acids Res. 32 1363–1371. 10.1093/nar/gkh293 PubMed DOI PMC

Man D., Wang W., Sabehi G., Aravind L., Post A. F., Massana R., et al. (2003). Diversification and spectral tuning in marine proteorhodopsins. EMBO J. 22 1725–1731. 10.1093/emboj/cdg183 PubMed DOI PMC

Marchler-Bauer A., Derbyshire M. K., Gonzales N. R., Lu S., Chitsaz F., Geer L. Y., et al. (2015). CDD: NCBI’s conserved domain database. Nucleic Acids Res. 43 D222–D226. 10.1093/nar/gku1221 PubMed DOI PMC

Mas A., Jamshidi S., Lagadeuc Y., Eveillard D., Vandenkoornhuyse P. (2016). Beyond the Black Queen hypothesis. ISME J. 10 2085–2091. 10.1038/ismej.2016.22 PubMed DOI PMC

Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. (2013). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform. 14:60. 10.1186/1471-2105-14-60 PubMed DOI PMC

Mongodin E. F., Nelson K. E., Daugherty S., Deboy R. T., Wister J., Khouri H., et al. (2005). The genome of Salinibacter ruber: converfence and gene exchange among hyperhalophilic bacteria and archaea. Proc. Natl. Acad. Sci. U.S.A. 102 18147–18152. 10.1073/pnas.0509073102 PubMed DOI PMC

Moore S. J., Warren M. J. (2012). The anaerobic biosynthesis of vitamin B12. Biochem. Soc. Trans. 40 581–586. 10.1042/BST20120066 PubMed DOI

Morris J. J. (2015). Black Queen evolution: the role of leakiness in structuring microbial communities. Trends Genet. 31 475–482. 10.1016/j.tig.2015.05.004 PubMed DOI

Morris J. J., Lenski R. E., Zinser E. R. (2012). The black queen hypothesis: evolution of dependencies through adaptive gene loss. mBio 3:e00036-12. 10.1128/mBio.00036-12 PubMed DOI PMC

Morris M. S. (2012). The role of B vitamins in preventing and treating cognitive impairment and decline. Adv. Nutr. 3 801–812. 10.3945/an.112.002535 PubMed DOI PMC

Nguyen L.-T., Schmidt H. A., von Haeseler A., Minh B. Q. (2015). IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 32 268–274. 10.1093/molbev/msu300 PubMed DOI PMC

Oesterhelt D., Stoeckenius W. (1973). Functions of a new photoreceptor membrane. Proc. Natl. Acad. Sci. U.S.A. 70 2853–2857. 10.1073/PNAS.70.10.2853 PubMed DOI PMC

Oesterhelt D., Stoecknius W. (1971). Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nat. New Biol. 233 149–152. 10.1038/newbio233149a0 PubMed DOI

Oren A. (2011). Thermodynamic limits to microbial life at high salt concentrations. Environ. Microbiol. 13 1908–1923. 10.1111/j.1462-2920.2010.02365.x PubMed DOI

Oren A. (2013). Life at high salt concentrations, intracellular KCl concentrations, and acidic proteomes. Front. Microbiol. 4:315. 10.3389/fmicb.2013.00315 PubMed DOI PMC

Oren A., Ventosa A., Grant W. D. (1997). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int. J. Syst. Bacteriol. 47 233–238. 10.1099/00207713-47-1-233 DOI

Parks D. H., Imelfort M., Skennerton C. T., Hugenholtz P., Tyson G. W. (2015). CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 25 1043–1055. 10.1101/gr.186072.114 PubMed DOI PMC

Patel R., Mevada V., Prajapati D., Dudhagara P., Koringa P., Joshi C. G. (2015). Metagenomic sequence of saline desert microbiota from wild ass sanctuary, Little Rann of Kutch, Gujarat, India. Genome Data 3 137–139. 10.1016/j.gdata.2015.01.003 PubMed DOI PMC

Pfeiffer F., Schuster S. C., Broicher A., Falb M., Palm P., Rodewald K., et al. (2008). Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1. Genomics 91 335–346. 10.1016/j.ygeno.2008.01.001 PubMed DOI

Plominsky A. M., Delherbe N., Ugalde J. A., Allen E. E., Blanchet M., Ikeda P., et al. (2014). Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at Cáhuil lagoon, Chile. Genome Announc. 2:e01172-14 10.1128/genomeA.01172-14 PubMed DOI PMC

Podell S., Emerson J. B., Jones C. M., Ugalde J. A., Welch S., Heidelberg K. B., et al. (2014). Seasonal fluctuations in ionic concentrations drive microbial succession in a hypersaline lake community. ISME J. 8 979–990. 10.1038/ismej.2013.221 PubMed DOI PMC

Podell S., Ugalde J. A., Narasingarao P., Banfield J. F., Heidelberg K. B., Allen E. E. (2013). Assembly-driven community genomics of a hypersaline microbial ecosystem. PLoS One 8:e61692. 10.1371/journal.pone.0061692 PubMed DOI PMC

Price M. N., Dehal P. S., Arkin A. P. (2010). FastTree 2 – approximately maximum-likelihood trees for large alignments. PLoS One 5:e9490. 10.1371/journal.pone.0009490 PubMed DOI PMC

Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., et al. (2007). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 35 7188–7196. 10.1093/nar/gkm864 PubMed DOI PMC

Rice P., Longden L., Bleasby A. (2000). EMBOSS: the european molecular biology open software suite. Trends Genet. 16 276–277. 10.1016/S0168-9525(00)02024-2 PubMed DOI

Richter M., Rosselló-Móra R. (2009). Shifting the genomic gold standard for the prokaryotic species definition. Proc. Natl. Acad. Sci. U.S.A. 106 19126–19131. 10.1073/pnas.0906412106 PubMed DOI PMC

Rodionov D. A., Vitreschak A. G., Mironov A. A., Gelfand M. S. (2003). Comparative genomics of the vitamin B12 metabolism and regulation in prokaryotes. J. Biol. Chem. 278 41148–41159. 10.1074/jbc.M305837200 PubMed DOI

Saum S. H., Pfeiffer F., Palm P., Rampp M., Schuster S. C., Müller V., et al. (2013). Chloride and organic osmolytes: a hybrid strategy to cope with elevated salinities by the moderately halophilic, chloride-dependent bacterium Halobacillus halophilus. Environ. Microbiol. 15 1619–1633. 10.1111/j.1462-2920.2012.02770.x PubMed DOI

Seemann T. (2014). Prokka: rapid prokaryotic genome annotation. Bioinformatics 30 2068–2069. 10.1093/bioinformatics/btu153 PubMed DOI

Stackebrandt E., Goebel B. M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 44 846–849. 10.1099/00207713-44-4-846 DOI

Subow N. N. (1931). Oceanographical Tables. Comissariat of agriculture of USSR. Hydro-Meteorogical Committee of USSR. Moscow: Oceanographical Institute of USSR.

Sun D.-L., Jiang X., Wu Q. L., Zhou N.-Y. (2013). Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity. Appl. Environ. Microbiol. 79 5962–5969. 10.1128/AEM.01282-13 PubMed DOI PMC

Talon R., Coquelle N., Madern D., Girard E. (2014). An experimental point of view on hydration/solvation in halophilic proteins. Front. Microbiol. 5:66. 10.3389/fmicb.2014.00066 PubMed DOI PMC

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30 2725–2729. 10.1093/molbev/mst197 PubMed DOI PMC

Tatusov R. L., Natale D. A., Garkavtsev I. V., Tatusova T. A., Shankavaram U. T., Rao B. S., et al. (2001). The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29 22–28. 10.1093/nar/29.1.22 PubMed DOI PMC

Torreblanca M., Rodríguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. (1986). Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst. Appl. Microbiol. 8 89–99. 10.1016/s0723-2020(86)80155-2 DOI

Ventosa A. (2006). “Unusual micro-organisms from unusual habitats: hypersaline environments,” in Prokaryotic Diversity: Mechanisms and Significance: Published for the Society for General Microbiology, eds Logan N. A., Lappin-Scott H. M., Oyston P. C. F. (Cambridge: Cambridge University Press; ), 223–254. 10.1017/CBO9780511754913.015 DOI

Ventosa A., de la Haba R. R., Sánchez-Porro C., Papke R. T. (2015). Microbial diversity of hypersaline environments: a metagenomic approach. Curr. Opin. Microbiol. 25 80–87. 10.1016/J.MIB.2015.05.002 PubMed DOI

Vera-Gargallo B., Chowdhury T. R., Brown J., Fansler S. J., Durán-Viseras A., Sánchez-Porro, et al. (2019). Spatial distribution of prokaryotic communities in hypersaline soils. Sci. Rep. 9:1769. 10.1038/s41598-018-38339-z PubMed DOI PMC

Vera-Gargallo B., Navarro-Sampedro L., Carballo M., Ventosa A. (2018). Metagenome sequencing of prokaryotic microbiota from two hypersaline soils of the odiel salt marshes in Huelva, Southwester Spain. Genome Announc. 6:e00140-18. 10.1128/genomeA.00140.18 PubMed DOI PMC

Vera-Gargallo B., Ventosa A. (2018). Metagenomic insights into the phylogenetic and metabolic diversity of the prokaryotic community dwelling in hypersaline soils from the Odiel Saltmarshes (SW Spain). Genes 9:E152. 10.3390/genes9030152 PubMed DOI PMC

Villarreal-Chiu J. F., Quinn J. P., McGrath J. W. (2012). The genes and enzymes of phosphonate metabolism by bacteria, and their distribution in the marine environment. Front. Microbiol. 3:19. 10.3389/fmicb.2012.00019 PubMed DOI PMC

Warden A. C., Williams M., Peat T. S., Seabrook S. A., Newman J., Dojchinov G., et al. (2015). Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst. Nat. Commun. 6:10278. 10.1038/ncomms10278 PubMed DOI PMC

Wood J. M., Bremer E., Csonka L. N., Kraemer R., Poolman B., van der Heide T., et al. (2001). Osmosensing and osmoregulatory compatible solute accumulation by bacteria. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 130 437–460. 10.1016/S1095-6433(01)00442-1 PubMed DOI

Woodson J. D., Peck R. F., Krebs M. P., Escalante-Semerena J. C. (2003). The cobY gene of the archaeon Halobacterium sp. strain NRC-1 is required for de novo Cobamide synthesis. J. Bacteriol. 185 311–316. 10.1128/JB.185.1.311-316.2003 PubMed DOI PMC

Youssef N. H., Savage-Ashlock K. N., McCully A. L., Luedtke B., Shaw E. I., Hoff W. D., et al. (2014). Trehalose/2-sulfotrehalose biosynthesis and glycine-betaine uptake are widely spread mechanisms for osmoadaptation in the Halobacteriales. ISME J. 8 636–649. 10.1038/ismej.2013.165 PubMed DOI PMC

Expanded Diversity and Metabolic Versatility of Marine Nitrite-Oxidizing Bacteria Revealed by Cultivation- and Genomics-Based Approaches

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