The objective of this work was to characterize the toxicological profile of a newly developed sunscreen formulation based on polymeric nanocapsules (NCs) loading benzophenone-3 (BZP3). NCs composed of poly(ε-caprolactone) carrot oil and Pluronic® F68 were produced by emulsification-diffusion method. Their mean particle size (Z-Ave) ranged from 280 to 420 nm, polydispersity index (PDI) was below 0.37, while zeta potential (ZP) reached about |+11 mV|. No cytotoxic effects were observed in L929 fibroblast cell line for the blank (i.e., non-loaded) NCs and BZP3-loaded NCs (BZP3-NCs). The semi-solid sunscreen formulation was stable over time (centrifugation testing) and exhibited non-Newtonian pseudoplastic behavior, which is typical of products for topical application onto the skin. The sun protection factor (SPF) value reached 8.84, when incorporating BZP3-NCs (SPF of 8.64) into the semi-solid formulation. A synergistic effect was also observed when combining the formulation ingredients of nanocapsules, i.e., SPF of carrot oil was 6.82, blank NCs was 6.84, and BZP3-loaded NCs was 8.64. From the hen's egg-chorioallantoic membrane test (HET-CAM) test, the non-irritation profile of the developed formulations could also be confirmed. The obtained results show a promising use of poly(ε-caprolactone) nanocapsules to be loaded with lipophilic sunscreens as benzophenone-3.

CEB Centre of Biological Engineering University of Minho Campus de Gualtar 4710 057 Braga Portugal

Department of Morphology Federal University of Sergipe Avenida Marechal Rondon São Cristovão 49100 000 Brazil

Department of Pharmaceutical Technology Faculty of Pharmacy University of Coimbra Pólo das Ciências da Saúde Azinhaga de Santa Comba 3000 548 Coimbra Portugal

Department of Pharmacy Pharmaceutical Technology and Physical Chemistry and Institute of Nanoscience and Nanotechnology Faculty of Pharmacy University of Barcelona 08028 Barcelona Spain

Institute of Macromolecular Chemistry Academy of Sciences of the Czech Republic v v i Heyrovsky Sq 2 162 06 Prague Czech Republic

Institute of Technology and Research Av Murilo Dantas 300 Aracaju 49010 390 Brazil

Tiradentes Institute 150 Mt Vernon St Dorchester MA 02125 USA

University of Tiradentes Biotechnological Postgraduate Program Av MuriloDantas 300 Aracaju 49010 390 Brazil

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Nanotechnology in Cosmetics and Cosmeceuticals-A Review of Latest Advancements