Infrared Absorption Spectroscopy of Albumin Binding with Amine-Containing Plasma Polymer Coatings on Nanoporous Diamond Surfaces
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Diamond chemistry MeSH
- Nanopores * MeSH
- Plasma Gases chemistry MeSH
- Surface Properties MeSH
- Serum Albumin, Bovine chemistry MeSH
- Cattle MeSH
- Spectrophotometry, Infrared MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Diamond MeSH
- Plasma Gases MeSH
- Serum Albumin, Bovine MeSH
Nanocrystalline diamond (NCD) layers functionalized with amine-containing functional groups have generated considerable interest as biocompatible substrates for attachment of biomolecules and cells with a view to biosensor and tissue engineering applications. Here we prepare nanoporous diamond layers with the surfaces modified by hydrogen plasma, oxygen plasma, and conformal 7 nm amine-containing plasma polymer (PP). Immobilization of bovine serum albumin (BSA) molecules is characterized on such surfaces. Grazing angle reflectance infrared spectroscopy as well as X-ray photoelectron spectroscopy show that concentration of amine-containing bonds after BSA exposure depends on the type of NCD surface modification. AFM measurements reveal that BSA proteins are physisorbed on H- and O-terminated diamond surfaces in different thicknesses and morphology. When the diamond layers are coated with the amine-containing PP, BSA molecules assume similar thickness and morphology, and their adhesion is significantly increased on both types of the diamond surfaces.
Faculty of Electrical Engineering Czech Technical University 166 27 Prague 6 Czech Republic
Faculty of Mathematics and Physics Charles University 18000 Prague 8 Czech Republic
Institute of Physics Czech Academy of Sciences 162 00 Prague 6 Czech Republic
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