Oxidative DNA Cleavage with Clip-Phenanthroline Triplex-Forming Oligonucleotide Hybrids
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
BB/J001694/2
Biotechnology and Biological Sciences Research Council - United Kingdom
BB/ R008655/1
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
31680391
DOI
10.1002/cbic.201900670
Knihovny.cz E-zdroje
- Klíčová slova
- DNA cleavage, click chemistry, copper, nucleases, oligonucleotides,
- MeSH
- click chemie MeSH
- deoxyribonukleasy chemie metabolismus MeSH
- DNA chemie metabolismus MeSH
- fenantroliny chemická syntéza chemie MeSH
- konformace nukleové kyseliny MeSH
- oligonukleotidy chemie MeSH
- sekvence nukleotidů MeSH
- štěpení DNA * MeSH
- tranzitní teplota MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- deoxyribonukleasy MeSH
- DNA MeSH
- fenantroliny MeSH
- oligonukleotidy MeSH
- triplex DNA MeSH Prohlížeč
A systematic study of several new types of hybrids of Cu-chelated clamped phenanthroline artificial metallonuclease (AMN) with triplex-forming oligonucleotides (TFO) for sequence-specific cleavage of double-stranded DNA (dsDNA) is reported. The synthesis of these AMN-TFO hybrids is based on application of the alkyne-azide cycloaddition click reaction as the key step. The AMN was attached through different linkers at either the 5'- or 3'-ends or in the middle of the TFO stretch. The diverse hybrids efficiently formed triplexes with the target purine-rich sequence and their copper complexes were studied for their ability to cleave dsDNA in the presence of ascorbate as a reductant. In all cases, the influence of the nature and length of the AMN-TFO, time, conditions and amounts of ascorbate were studied, and optimum conjugates and a procedure that gave reasonably efficient (up to 34 %) cleavage of the target sequence, while rendering an off-target dsDNA intact, were found. The footprint of cleavage on PAGE was identified only in one case, with low conversion; this means that cleavage does not proceed with single nucleotide precision. On the other hand, these AMN-TFO hybrids are useful for the selective degradation of target dsDNA sequences. Future improvements to this design may provide higher resolution and selectivity.
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