Coumarin Tröger's base derivatives with cyanine substitution as selective and sensitive fluorescent lysosomal probes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31810756
DOI
10.1016/j.bioorg.2019.103447
PII: S0045-2068(19)30777-1
Knihovny.cz E-resources
- Keywords
- Coumarin, Cyanine dye, Fluorescent probe, Lysosomal probe, Tröger’s base,
- MeSH
- Fluorescent Dyes chemical synthesis chemistry MeSH
- Microscopy, Fluorescence MeSH
- Carbocyanines chemistry MeSH
- Cells, Cultured MeSH
- Coumarins chemical synthesis chemistry MeSH
- Humans MeSH
- Lysosomes chemistry MeSH
- Molecular Structure MeSH
- Optical Imaging * MeSH
- Dose-Response Relationship, Drug MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Carbocyanines MeSH
- Coumarins MeSH
The fluorescent probes based on Tröger's base motive with both coumarin and cyanine substitution 11-13 have been synthesized by multi-step synthesis in high overall yields. Intracellular localization of prepared probes have been tested using four different cell lines (HF-P4, BLM, U-2 OS and A-2058). Prepared probes have intensive green and red fluorescence. Co-localization with commercial lysosome specific marker LysoTracker Blue DND 22 has been confirmed that all prepared fluorescent probes labeled lysosomal compartment with high selectivity and probes show excellent brightness at low concentration.
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