Juglans regia L. (English walnut) trees with cankers and dieback symptoms were observed in two regions in the Czech Republic. Isolations were made from diseased branches. In total, 138 fungal isolates representing 10 fungal species were obtained from wood samples and identified based on morphological characteristics and molecular methods: Cadophora novi-eboraci, Cadophora spadicis, Cryptovalsa ampelina, Diaporthe eres, Diplodia seriata, Dothiorella omnivora, Eutypa lata, Eutypella sp., Peroneutypa scoparia, and Phaeoacremonium sicilianum. Pathogenicity tests conducted under field conditions with all species using the mycelium-plug method indicated that Eutypa lata and Cadophora spp. were highly virulent to woody stems of walnut. This is the first study to detect and identify fungal trunk pathogens associated with diseased walnut trees in Europe.

Department of Fruit Growing Faculty of Horticulture Mendel University in Brno 69144 Lednice Czech Republic

Instituto Agroforestal Mediterráneo Universitat Politècnica de València 46022 Valencia Spain

Instituto de Ciencias de la Vid y del Vino Consejo Superior de Investigaciones Científicas Universidad de la Rioja Gobierno de La Rioja 26007 Logroño Spain

Mendeleum Institute of Genetics Faculty of Horticulture Mendel University in Brno 69144 Lednice Czech Republic

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