In this work we discuss a new label-free biosensing device based on indium tin oxide (ITO) overlaid section of a multimode optical fiber fused silica core. The sensor has been used to optical measurements also simultaneously interrogated electrochemically (EC). Due to optimized thickness and optical properties of ITO film, a lossy-mode resonance (LMR) could be observed in the optical domain, where electrical properties of the film allowed for application of the sensor as a working electrode in an EC setup. It has been confirmed that the LMR response depends on optical properties of the external medium, as well as potential applied to the electrode during cyclic voltammetry. After the ITO surface functionalization with amine groups and covalently attached biotin, the device has been applied for label-free biosensing of avidin in both the domains simultaneously. On the example of biotin-avidin detection system it was demonstrated that when avidin concentration increases a decrease in current and increase in LMR wavelength shift were recorded in EC and optical domain, respectively. Both optical and EC responses follow the protein interaction process, and thus can be used as cross-verification of the readouts. Moreover, an extended information has been achieved comparing to solely EC interrogation, i.e., the grafting process of biotin and avidin was directly monitored optically displaying individual steps of an incubation procedure.

Gdansk University of Technology Faculty of Electronics Telecommunications and Informatics Narutowicza 11 12 80 233 Gdańsk Poland

Polish Academy of Sciences Institute of Physical Chemistry Kasprzaka 44 52 01 224 Warszawa Poland

University of South Bohemia Institute of Physics Branisovska 1760 370 05 Ceske Budejovice Czech Republic

Warsaw University of Technology Institute of Microelectronics and Optoelectronics Koszykowa 75 00 662 Warszawa Poland

Warsaw University of Technology Institute of Microelectronics and Optoelectronics Koszykowa 75 00 662 Warszawa Poland; National Institute of Telecommunications Szachowa 1 04 894 Warszawa Poland

References provided by Crossref.org

Electro-optically modulated lossy-mode resonance