RNA-Binding Proteins and Their Targets in Trypanosoma brucei: Single Nucleotide Resolution Using iCLIP and iCLAP
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
R01 GM084065
NIGMS NIH HHS - United States
PubMed
32221928
PubMed Central
PMC7224312
DOI
10.1007/978-1-0716-0294-2_19
Knihovny.cz E-resources
- Keywords
- Posttranscriptional gene regulation, RNA-binding proteins (RBPs), iCLAP, iCLIP,
- MeSH
- Immunoprecipitation methods MeSH
- Nucleotides genetics metabolism MeSH
- Parasitology methods MeSH
- RNA-Binding Proteins analysis genetics metabolism MeSH
- Protozoan Proteins analysis genetics metabolism MeSH
- RNA, Protozoan genetics metabolism MeSH
- RNA genetics metabolism MeSH
- Trypanosoma brucei brucei genetics MeSH
- Ultraviolet Rays MeSH
- Protein Binding genetics radiation effects MeSH
- Binding Sites genetics MeSH
- Single Molecule Imaging methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nucleotides MeSH
- RNA-Binding Proteins MeSH
- Protozoan Proteins MeSH
- RNA, Protozoan MeSH
- RNA MeSH
RNA-binding proteins (RBPs) are critical to posttranscriptional gene regulation. Therefore, characterization of the RNA molecules bound by RBPs in vivo represent a key step in elucidating their function. The recently developed iCLIP technique allows single nucleotide resolution of the RNA binding footprints of RBPs. We present the iCLIP technique modified for its application to Trypanosoma brucei and most likely other kinetoplastid flagellates. By using the immuno- or affinity purification approach, it was successfully applied to the analysis of several RBPs. Furthermore, we also provide a detailed description of the iCLIP/iCLAP protocol that shall be particularly suitable for the studies of trypanosome RBPs.
Department of Microbiology The Ohio State University Columbus OH USA
Faculty of Science University of South Bohemia České Budějovice Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
The Center for RNA Biology The Ohio State University Columbus OH USA
The Ohio State Biochemistry Program The Ohio State University Columbus OH USA
See more in PubMed
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