RNA-Binding Proteins and Their Targets in Trypanosoma brucei: Single Nucleotide Resolution Using iCLIP and iCLAP
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
R01 GM084065
NIGMS NIH HHS - United States
PubMed
32221928
PubMed Central
PMC7224312
DOI
10.1007/978-1-0716-0294-2_19
Knihovny.cz E-zdroje
- Klíčová slova
- Posttranscriptional gene regulation, RNA-binding proteins (RBPs), iCLAP, iCLIP,
- MeSH
- imunoprecipitace metody MeSH
- nukleotidy genetika metabolismus MeSH
- parazitologie metody MeSH
- proteiny vázající RNA analýza genetika metabolismus MeSH
- protozoální proteiny analýza genetika metabolismus MeSH
- RNA protozoální genetika metabolismus MeSH
- RNA genetika metabolismus MeSH
- Trypanosoma brucei brucei genetika MeSH
- ultrafialové záření MeSH
- vazba proteinů genetika účinky záření MeSH
- vazebná místa genetika MeSH
- zobrazení jednotlivé molekuly metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nukleotidy MeSH
- proteiny vázající RNA MeSH
- protozoální proteiny MeSH
- RNA protozoální MeSH
- RNA MeSH
RNA-binding proteins (RBPs) are critical to posttranscriptional gene regulation. Therefore, characterization of the RNA molecules bound by RBPs in vivo represent a key step in elucidating their function. The recently developed iCLIP technique allows single nucleotide resolution of the RNA binding footprints of RBPs. We present the iCLIP technique modified for its application to Trypanosoma brucei and most likely other kinetoplastid flagellates. By using the immuno- or affinity purification approach, it was successfully applied to the analysis of several RBPs. Furthermore, we also provide a detailed description of the iCLIP/iCLAP protocol that shall be particularly suitable for the studies of trypanosome RBPs.
Department of Microbiology The Ohio State University Columbus OH USA
Faculty of Science University of South Bohemia České Budějovice Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
The Center for RNA Biology The Ohio State University Columbus OH USA
The Ohio State Biochemistry Program The Ohio State University Columbus OH USA
Zobrazit více v PubMed
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