Melanoma xenotransplant on the chicken chorioallantoic membrane: a complex biological model for the study of cancer cell behaviour
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
CNE reg. no. CZ.02.1.01/0.0/0.0/16_019/0000785
Ministerstvo Školství, Mládeže a Tělovýchovy (CZ)
Project BIOCEV-FAR reg. no. LQ1604
Ministerstvo Školství, Mládeže a Tělovýchovy
CZ.1.05/1.1.00/02.0109
Ministerstvo Školství, Mládeže a Tělovýchovy
reg. No. CZ.1.05/2.1.00/19.0400
RDIOP
PROGRES Q28
Univerzita Karlova v Praze
PubMed
32232553
DOI
10.1007/s00418-020-01872-y
PII: 10.1007/s00418-020-01872-y
Knihovny.cz E-resources
- Keywords
- Cancer microenvironment, Cancer-associated fibroblasts, Chorioallantoic membrane, Embryo, Melanoma, Melanoma invasiveness,
- MeSH
- Models, Biological * MeSH
- Chorioallantoic Membrane metabolism pathology MeSH
- Immunohistochemistry MeSH
- Chickens MeSH
- Chick Embryo MeSH
- Humans MeSH
- Melanoma, Experimental metabolism pathology MeSH
- Tumor Cells, Cultured MeSH
- Animals MeSH
- Check Tag
- Chick Embryo MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The globally increasing incidence of cancer, including melanoma, requires novel therapeutic strategies. Development of successful novel drugs is based on clear identification of the target mechanisms responsible for the disease progression. The specific cancer microenvironment represents a critically important aspect of cancer biology, which cannot be properly studied in simplistic cell culture conditions. Among other traditional options, the study of melanoma cell growth on the chicken chorioallantoic membrane offers several significant advantages. This model offers increased complexity compared to usual in silico culture models and still remains financially affordable. Using this model, we studied the growth of three established human melanoma cell lines: A2058, BLM, G361. The combination of histology, immunohistochemistry with the application of human-specific antibodies, intravascular injection of contrast material such as filtered Indian ink, Mercox solution and phosphotungstic acid, and X-ray micro-CT and live-cell monitoring was employed. Melanoma cells spread well on the chicken chorioallantoic membrane. However, invasion into the stroma of the chorioallantoic membrane and the limb primordium graft was rare. The melanoma cells also significantly influenced the architecture of the blood vessel network, resulting in the orientation of the vessels to the site of the tumour cell inoculation. The system of melanoma cell culture on the chorioallantoic membrane is suitable for the study of melanoma cell growth, particularly of rearrangement of the host vascular pattern after cancer cell implantation. The system also has promising potential for further development.
BIOCEV 1st Faculty of Medicine Charles University 25250 Vestec Czech Republic
Department of Stomatology 1st Faculty of Medicine Charles University 12800 Prague Czech Republic
Institute of Anatomy 1st Faculty of Medicine Charles University 12800 Prague Czech Republic
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