The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled
Jazyk angličtina Země Velká Británie, Anglie Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32366678
DOI
10.1242/dev.186338
PII: dev.186338
Knihovny.cz E-zdroje
- Klíčová slova
- Cadherin-11, Dishevelled, GEF Trio, Neural crest cell migration, Rho GTPases, Xenopus,
- MeSH
- crista neuralis cytologie embryologie MeSH
- fenotyp MeSH
- HEK293 buňky MeSH
- lidé MeSH
- plazmidy genetika MeSH
- pohyb buněk genetika MeSH
- protein dishevelled genetika metabolismus MeSH
- protein-serin-threoninkinasy genetika metabolismus MeSH
- proteinové domény MeSH
- proteiny Xenopus genetika metabolismus MeSH
- rac1 protein vázající GTP metabolismus MeSH
- rhoA protein vázající GTP metabolismus MeSH
- signální transdukce genetika MeSH
- transfekce MeSH
- vazba proteinů genetika MeSH
- výměnné faktory guaninnukleotidů genetika metabolismus MeSH
- Xenopus laevis embryologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DVL1 protein, Xenopus MeSH Prohlížeč
- DVL2 protein, human MeSH Prohlížeč
- Dvl2 protein, Xenopus MeSH Prohlížeč
- protein dishevelled MeSH
- protein-serin-threoninkinasy MeSH
- proteiny Xenopus MeSH
- rac1 protein vázající GTP MeSH
- rhoA protein vázající GTP MeSH
- TRIO protein, human MeSH Prohlížeč
- výměnné faktory guaninnukleotidů MeSH
Directional migration during embryogenesis and tumor progression faces the challenge that numerous external signals need to converge to precisely control cell movement. The Rho guanine exchange factor (GEF) Trio is especially well suited to relay signals, as it features distinct catalytic domains to activate Rho GTPases. Here, we show that Trio is required for Xenopus cranial neural crest (NC) cell migration and cartilage formation. Trio cell-autonomously controls protrusion formation of NC cells and Trio morphant NC cells show a blebbing phenotype. Interestingly, the Trio GEF2 domain is sufficient to rescue protrusion formation and migration of Trio morphant NC cells. We show that this domain interacts with the DEP/C-terminus of Dishevelled (DVL). DVL - but not a deletion construct lacking the DEP domain - is able to rescue protrusion formation and migration of Trio morphant NC cells. This is likely mediated by activation of Rac1, as we find that DVL rescues Rac1 activity in Trio morphant embryos. Thus, our data provide evidence for a novel signaling pathway, whereby Trio controls protrusion formation of cranial NC cells by interacting with DVL to activate Rac1.
Department of Experimental Biology Faculty of Science Masaryk University Brno 62500 Czech Republic
Max Delbrück Center for Molecular Medicine in the Helmholtz Association 13125 Berlin Germany
Philipps Universität Marburg Faculty of Biology Molecular Embryology 35043 Marburg Germany
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