Polymorphism of the PLIN1 gene and its association with body measures and ultrasound carcass traits in Qinchuan beef cattle
Language English Country Canada Media print-electronic
Document type Journal Article
PubMed
32615043
DOI
10.1139/gen-2019-0184
Knihovny.cz E-resources
- Keywords
- PLIN1 gene, Qinchuan cattle, body measurement traits, bovins Qinchuan, caractères de la morphologie corporelle, expression de l’ARNm, gène PLIN1, mRNA expression, marker-assisted selection, polymorphismes mononucléotidiques, single nucleotide polymorphisms, sélection assistée par marqueurs,
- MeSH
- Alleles MeSH
- Gene Expression MeSH
- Phenotype MeSH
- Genetic Variation MeSH
- Genetic Association Studies MeSH
- Genotype MeSH
- Haplotypes MeSH
- Polymorphism, Single Nucleotide MeSH
- Quantitative Trait, Heritable * MeSH
- Quantitative Trait Loci MeSH
- Perilipin-1 genetics MeSH
- Polymorphism, Genetic * MeSH
- Amino Acid Sequence MeSH
- Cattle MeSH
- Body Weights and Measures * MeSH
- Linkage Disequilibrium MeSH
- Body Size MeSH
- Computational Biology methods MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Perilipin-1 MeSH
The PLIN1 gene produces a phosphorylated protein wrapped in lipid droplets in adipocytes. This phosphorylation assists the mobilization of fat into adipose tissue. The purpose of the experiment was to study the polymorphism of the PLIN1 gene and its relationship with the body and carcass characteristics of Qinchuan cattle to find molecular genetic markers that can be used for breeding. The expression level of the PLIN1 gene was determined in various tissues by qRT-PCR. The results showed that the highest level of PLN1 expression was found in subcutaneous fat, followed by the heart and longissimus muscle, and the lowest level was found in the kidney. Five SNP loci of the PLIN1 gene were identified in 510 Qinchuan cattle, including g.3580T>C (SNP1), g.3898G>A (SNP2), g.8333G>A (SNP3), g.10517T>C (SNP4), and g.10538G>T (SNP5). The results show that SNP1, SNP2, SNP3, and SNP4 were moderately polymorphic (0.25 < PIC < 0.5), while SNP5 was minimally polymorphic (PIC < 0.25). SNP2, SNP3, and SNP5 were within Hardy-Weinberg equilibrium (P > 0.05), but SNP1 and SNP4 were not (P < 0.05). Correlation analysis showed that the five SNPs of the PLIN1 gene were correlated with back-fat depth, intramuscular fat, and chest depth of Qinchuan cattle. The double haplotype H2H4 in Qinchuan beef was associated with body and carcass traits. We conclude that variants mapped within PLIN1 can be used in marker-assisted selection for carcass quality and body traits in breed improvement programs for Qinchuan cattle.
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