Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32950875
DOI
10.1016/j.bpc.2020.106475
PII: S0301-4622(20)30183-6
Knihovny.cz E-zdroje
- Klíčová slova
- BSA, CeO(2), TiO(2), Zeta potential, ZnO,
- MeSH
- cer chemie MeSH
- nanočástice chemie MeSH
- oxid zinečnatý chemie MeSH
- povrchové vlastnosti MeSH
- sérový albumin hovězí chemie MeSH
- skot MeSH
- titan chemie MeSH
- velikost částic MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cer MeSH
- ceric oxide MeSH Prohlížeč
- oxid zinečnatý MeSH
- sérový albumin hovězí MeSH
- titan MeSH
- titanium dioxide MeSH Prohlížeč
This article aims to explore interaction of nanoparticles ZnO, TiO2 and CeO2 with bovine serum albumin (BSA). The samples of nanoparticles were laboratory prepared and compared with commercial nanoparticles. Nanoparticles were characterized by SEM, zeta potential and size measurements. Adsorption of particles took place at pH where the zeta potential of the protein and nanoparticles was opposite. Then the zeta potential and size distribution were measured and the amount of protein needed to reduce the zeta potential of nanoparticles to zero was determined. The changes of BSA structure were also observed by Circular Dichroism (CD). The change of BSA structure after the adsorption on nanoparticles was confirmed. The content of BSA α-helix structure varies during experiments from 13 to 77% in dependence of concentration of nanoparticles. The interaction of TiO2 and BSA was confirmed also by the Surface Plasmon Resonance (SPR) technique. On laboratory prepared TiO2 was bound amount of 73,4 pg·mm-2.
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