Staining Performance of ALK and ROS1 Immunohistochemistry and Influence on Interpretation in Non-Small-Cell Lung Cancer
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
33011443
DOI
10.1016/j.jmoldx.2020.09.006
PII: S1525-1578(20)30482-7
Knihovny.cz E-zdroje
- MeSH
- anaplastická lymfomová kináza metabolismus MeSH
- hybridizace in situ fluorescenční metody MeSH
- imunohistochemie metody MeSH
- laboratoře nemocniční MeSH
- lidé MeSH
- nádory plic metabolismus MeSH
- nemalobuněčný karcinom plic metabolismus MeSH
- patologové MeSH
- protoonkogen Mas MeSH
- protoonkogenní proteiny metabolismus MeSH
- senzitivita a specificita MeSH
- tyrosinkinasy metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ALK protein, human MeSH Prohlížeč
- anaplastická lymfomová kináza MeSH
- MAS1 protein, human MeSH Prohlížeč
- protoonkogen Mas MeSH
- protoonkogenní proteiny MeSH
- ROS1 protein, human MeSH Prohlížeč
- tyrosinkinasy MeSH
Selection of non-small-cell lung cancer patients for treatment relies on the detection of expression of anaplastic lymphoma kinase (ALK) and ROS proto-oncogene 1 (ROS1) protein by immunohistochemistry (IHC). We evaluated staining performance for different IHC protocols and laboratory characteristics, and their influence on ALK and ROS1 interpretation during external quality assessment schemes between 2015 and 2018. Participants received five formalin-fixed, paraffin-embedded cases for staining by their routine protocol, whereafter at least two pathologists scored them simultaneously under a multihead microscope and awarded a graded expert staining score (ESS) from 1 to 5 points based on staining quality. European Conformity in Vitro Diagnostic kits (such as D5F3) revealed a better ALK ESS compared with laboratory-developed tests. ESS was indifferent to the applied antibody dilution or a recent protocol change. Lower ESSs were observed for higher antibody incubation times and temperatures. ESS for various ROS1 protocols were largely similar. Overall, for both markers, ESS improved over time and for repeated external quality assessment participation but was independent of laboratory setting or experience. Except for ROS1, ESS positively correlated with laboratory accreditation. IHC stains with lower ESS correlated with increased error rates in ALK and ROS1 interpretation and analysis failures. Laboratory characteristics differently affected staining quality and interpretation, and laboratories should assess both aspects, and less common protocols need improvement in staining performance.
Department of Pathology Charles University Medical Faculty Hospital Hradec Kralove Czech Republic
Department of Pathology Erasmus Medical Center Rotterdam Rotterdam the Netherlands
Department of Pathology Vrije Universiteit Amsterdam Medical Center Amsterdam the Netherlands
Citace poskytuje Crossref.org
