Use of nanoparticles (NPs) in several commercial products has led to emergence of novel contaminants of air, soil and water bodies. The NPs may exhibit greater ecotoxicity due to nano-scale dependent properties over their bulk counterparts. The present investigation explores the effect of in vitro supplementation of TiO2, silica and silver NPs on radial growth and ultrastructural changes in the hyphae and spores of two mushroom genera, Ganoderma lucidum and Volvariella volvaceae. A concentration dependent decrease in radial growth on NP amended potato dextrose agar medium was recorded. However, in comparison to control, there was decrease in radial diameter on supplementation with TiO2 NPs while an increase was recorded for silica and silver NPs amendments as compared to their bulk salts at same concentrations after 48 h of incubation. Optical microscopy studies showed decrease in the number of spores while increase in spore diameter and thinning of hyphal diameter on NPs supplementation. Scanning electron microscopy analysis of fungal growth showed presence of deflated and oblong spores in two fruiting strains of Ganoderma while Volvariella exhibited decreased sporulation. Further, hyphal thinning and branching was recorded in response to NP amendments in both the test mushrooms. Enhancement of protein content was observed on NP compared to bulk supplementation for all cultures, concentrations and hours of incubation except for TiO2 NPs. Likewise, bulk and NP supplementations (at 100 mg L -1) resulted in enhanced laccase activity with occurrence of laccase specific protein bands on SDS-PAGE analysis.

College of Basic Sciences and Humanities Punjab Agricultural University Ludhiana India

Department of Chemistry Faculty of Science University of Hradec Kralove Hradec Kralove Czech Republic

Electron Microscopy and Nanoscience Laboratory Department of Soil Science College of Agriculture Punjab Agricultural University Ludhiana India

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Khan I, Saeed K, Khan I.. Nanoparticles: properties, applications and toxicities. Arabian J Chem. 2019;12(7):908–931.

Tratnyek PG, Johnson RL.. Nanotechnologies for environmental cleanup. Nanotoday. 2006;1(2):44–48.

Inshakova E, Inshakov O.. World market for nanomaterials: structure and trends. MATEC Web Conf ICMTMTE. 2017;129:02013.

Jeevanandam J, Barhoum A, Chan YS, et al. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018;9:1050–1074. PubMed PMC

Maynard AD, Aitken RJ, Butz T, et al. Safe handling of nanotechnology. Nature. 2006;444(7117):267–269. PubMed

Huan C, Shu-Qing S.. Silicon nanoparticles: preparation, properties, and applications. Physical Biol. 2015;46(39):no–14.

Hashimoto K, Irie H, Fujishima A.. TiO

Fujioka K, Hiruoka M, Sato K, et al. Luminescent passive-oxidized silicon quantum dots as biological staining labels and their cytotoxicity effects at high concentration. Nanotechnol. 2008;19(41):1–7. PubMed

Wiesner MR, Lowry GV, Alvarez P, et al. Assessing the risks of manufactured nanomaterials. Environ Sci Technol. 2006;40(14):4336–4345. PubMed

Baldrian P. Wood-inhabiting ligninolytic basidiomycetes in soils: ecology and constraints for applicability in bioremediation. Fungal Ecol. 2008;1(1):4–12.

Gianfreda L, Xu F, Bollag JM.. Laccases: a useful group of oxidoreductive enzymes. Bioremediation J. 1999;3(1):1–26.

P. Baldrian. Effect of heavy metals on saprotrophic soil fungi In: Sherameti I, Varma A, editors. Soil heavy metals. Berlin (Heidelberg): Springer-Verlag; 2010. p. 263–279.

Amdekar S.

Bozzola JJ, Russell LD.. Electron microscopy: principles and techniques for biologists. Boston; Jones & Bartlett; 1999.

Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–275. PubMed

Laemmli UK, Molbert E, Showe M, et al. Form-determining function of the genes required for the assembly of the head of bacteriophage T4. J Mol Biol. 1970;49(1):99–113. PubMed

Turner EM. Phenoloxidase activity in relation to substrate and developmental stage in mushroom

Singh RP, Garcha HS, Khanna PK.. Laccase production by

Dhaliwal MS, Jindal SK, Dhaliwal LK, et al. Growth and yield of tomato influenced by condition of culture, mulch and planting date. Intl J Veg Sci. 2017;23(1):4–17.

Goyal A, Kalia A, Sodhi HS.. Selenium stress in

Baldrian P. Fungal laccases – occurrence and properties. FEMS Microbiol Rev. 2006;30(2):215–242. PubMed

Baldrian P. Interactions of heavy metals with white-rot fungi. Enzyme Microb Technol. 2003;32(1):78–91.

Galhaup C, Haltrich D.. Enhanced formation of laccase activity by the white-rot fungus PubMed

Tychanowicz GK, de Souza DF, Souza CGM, et al. Copper improves the production of laccase by the white-rot fungus

Baldrian P, Gabriel J.. Copper and cadmium increase laccase activity in PubMed

Guo S, Li H, Yang J, et al. Visible-light-induced effects of Au nanoparticle on laccase catalytic activity. ACS Appl Mater Interfaces. 2015;7(37):20937–20944. PubMed