RNA of Kyzylagach virus (KYZV), a Sindbis-like mosquito-borne alphavirus from Western equine encephalitis virus complex, was detected in four pools (out of 221 pools examined), encompassing 10,784 female Culex modestus mosquitoes collected at a fishpond in south Moravia, Czech Republic, with a minimum infection rate of 0.04%. This alphavirus was never detected in Central Europe before.

Institute of Vertebrate Biology The Czech Academy of Sciences v v i Květná 8 CZ 60365 Brno Czech Republic

Veterinary Research Institute Hudcova 70 CZ 62100 Brno Czech Republic

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Detection of Usutu virus in a house martin bug Oeciacus hirundinis (Hemiptera: Cimicidae): implications for virus overwintering in a temperate zone

History of Arbovirus Research in the Czech Republic