Entomological study in an anthroponotic cutaneous leishmaniasis focus in Morocco: Fauna survey, Leishmania infection screening, molecular characterization and MALDI-TOF MS protein profiling of relevant Phlebotomus species
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
Institut Pasteur du Maroc
778298
H2020-MSCA-RAISE-2017
PubMed
33686765
DOI
10.1111/tbed.14064
Knihovny.cz E-zdroje
- Klíčová slova
- Morocco, cutaneous leishmaniasis, leishmania tropica, molecular screening, proteomic identification, sand fly,
- MeSH
- hmyz - vektory MeSH
- kinetoplastová DNA MeSH
- Leishmania * genetika MeSH
- leishmanióza kožní * epidemiologie veterinární MeSH
- Phlebotomus * MeSH
- Psychodidae * MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Maroko epidemiologie MeSH
- Názvy látek
- kinetoplastová DNA MeSH
In Morocco, leishmaniases are a major public health problem due to their genetic diversity and geographical distribution. Cutaneous leishmaniasis (CL) is an infectious disease caused by various species of Leishmania and transmitted typically by bite of phlebotomine sand flies. This study identifies sand fly fauna in Ibaraghen village, province of Azilal, which is a focus of CL, by combination of morphological and molecular methods (sequencing of COI gene, MALDI-TOF MS protein profiling). Nested-kDNA PCR was used to detect and identify Leishmania species within potential vector species. 432 CDC light traps were placed at different heights above ground level at four capture sites during a whole year. Traps at 1.5 m above the ground yielded capture of sand flies almost double compared to above ground level (29.33%), while the collection reached 55.09% when the traps were placed 2.5 m above ground. A total of 2,830 sand flies were collected, 2,213 unfed specimens were morphologically identified, 990 males (44.73%) and 1,223 females (55.26%) of 13 species; ten Phlebotomus species and three Sergentomyia species. Six species were analysed by MALDI-TOF MS protein profiling (4 Phlebotomus and 2 Sergentomiya species), and their identification was confirmed by COI sequencing. 1,375 unfed females were screened for the presence of Leishmania by nested-kDNA PCR in pools, 11/30 pools of P. sergenti showing a single band of 750 bp corresponding to L. tropica. Our results confirm the role of P. sergenti as a proven vector in Azilal focus of cutaneous leishmaniasis; however, the relative abundance of other species known as vectors of Leishmania species emphasizes the risk of introduction of L. infantum and L. major in this province. For the first time in Morocco, a combined approach to identify sand flies by both morphology and molecular methods based on DNA barcoding and MALDI-TOF MS protein profiling was applied.
BioCeV Institute of Microbiology of the Czech Academy of Sciences Vestec Czech Republic
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Laboratory of Parasitology and Vector Borne Diseases Institut Pasteur du Maroc Casablanca Morocco
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RefSeq
MT231536, MT237926, MT233398, MT237928, MT234394, MT237929, MT237933, MT232346, MT232345, MT233401, MT237931, MT232344, MT231537, MT240843, MT232343, MT231534, MT231535, MT234393, MT240845, MT231533, MT231531, MT240844, MT240854, MT240853