A copper nanoparticle-based electrochemical immunosensor for carbaryl detection
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
33773707
DOI
10.1016/j.talanta.2021.122174
PII: S0039-9140(21)00095-3
Knihovny.cz E-resources
- Keywords
- Antibodies, Carbaryl, Copper nanoparticles, Electrochemical immunosensor, Gold-graphite electrode, Linear sweep anodic stripping voltammetry,
- MeSH
- Biosensing Techniques * MeSH
- Electrochemical Techniques MeSH
- Immunoassay MeSH
- Carbaryl MeSH
- Metal Nanoparticles * MeSH
- Limit of Detection MeSH
- Copper MeSH
- Nanoparticles * MeSH
- Gold MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Carbaryl MeSH
- Copper MeSH
- Gold MeSH
A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive determination of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of the GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 μg·kg-1, with a limit of detection 0.08 μg·kg-1. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks, and soil samples.
References provided by Crossref.org
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