Cronobacter sakazakii is a pathogen that causes severe diseases such as meningitis and necrotizing enterocolitis in infants under 12 months, associated with the consumption of contaminated rehydrated powdered infant formula (PIF). We present seven C. sakazakii genome sequences isolated from PIF and dairy products in Chile in 2017.

Austrian Agency for Health and Food Safety Institute for Medical Microbiology and Hygiene Vienna Austria

Department of Nutrition and Public Health Universidad del Bío Bío Chillán Chile

Department of Public Health Palacký University Olomouc Olomouc Czech Republic

foodmicrobe com Adams Hill Keyworth Nottinghamshire United Kingdom

Ñuble Health Service Ministry of Health Chillán Chile

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Tall BD, Chen Y, Yan Q, Gopinath GR, Grim CJ, Jarvis KG, Fanning S, Lampel KA. 2014. Cronobacter: an emergent pathogen causing meningitis to neonates through their feeds. Sci Prog 97:154–172. doi:10.3184/003685014X13994743930498. PubMed DOI PMC

Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H. 2008. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int J Syst Evol Microbiol 58:1442–1447. doi:10.1099/ijs.0.65577-0. PubMed DOI

Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ. 2012. Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. Int J Syst Evol Microbiol 62:1277–1283. doi:10.1099/ijs.0.032292-0. PubMed DOI

Forsythe SJ. 2018. Updates on the Cronobacter genus. Annu Rev Food Sci Technol 9:23–44. doi:10.1146/annurev-food-030117-012246. PubMed DOI

Parra-Flores J, Maury-Sintjago E, Rodriguez-Fernández A, Acuña S, Cerda F, Aguirre J, Holy O, 2020. Microbiological quality of powdered infant formula in Latin America. J Food Prot 83:534–541. doi:10.4315/0362-028X.JFP-19-399. PubMed DOI

Parra-Flores J, Cerda-Leal F, Contreras A, Valenzuela-Riffo N, Rodriguez A, Aguirre J. 2018. Cronobacter sakazakii and microbiological parameters in dairy formulas associated with a food alert in Chile. Front Microbiol 9:1708. doi:10.3389/fmicb.2018.01708. PubMed DOI PMC

Lepuschitz S, Ruppitsch W, Pekard-Amenitsch S, Forsythe SJ, Cormican M, Mach RL, Piérard D, Allerberger F, the EUCRONI Study Group . 2019. Multicenter study of Cronobacter sakazakii infections in humans, Europe, 2017. Emerg Infect Dis 25:515–522. doi:10.3201/eid2503.181652. PubMed DOI PMC

Bolger AM, Lohse M, Usadel B. 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. doi:10.1093/bioinformatics/btu170. PubMed DOI PMC

Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:10.1089/cmb.2012.0021. PubMed DOI PMC

Zhang Z, Schwartz S, Wagner L, Miller W. 2000. A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214. doi:10.1089/10665270050081478. PubMed DOI

Ondov BD, Treangen TJ, Melsted P, Mallonee AB, Bergman NH, Koren S, Phillippy AM. 2016. Mash: fast genome and metagenome distance estimation using MinHash. Genome Biol 17:132. doi:10.1186/s13059-016-0997-x. PubMed DOI PMC

Jolley KA, Bliss CM, Bennett JS, Bratcher HB, Brehony C, Colles FM, Wimalarathna H, Harrison OB, Sheppard SK, Cody AJ, Maiden MCJ. 2012. Ribosomal multilocus sequence typing: universal characterization of bacteria from domain to strain. Microbiology (Reading) 158:1005–1015. doi:10.1099/mic.0.055459-0. PubMed DOI PMC

Baldwin A, Loughlin M, Caubilla-Barron J, Kucerova E, Manning G, Dowson C, Forsythe S. 2009. Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes. BMC Microbiol 9:223. doi:10.1186/1471-2180-9-223. PubMed DOI PMC

Holý O, Parra-Flores J, Lepuschitz S, Alarcón-Lavín MP, Cruz-Córdova A, Xicohtencatl-Cortes J, Mancilla-Rojano J, Ruppitsch W, Forsythe S. 2020. Molecular characterization of Cronobacter sakazakii strains isolated from powdered milk. Foods 10:20. doi:10.3390/foods10010020. PubMed DOI PMC

Feldgarden M, Brover V, Haft DH, Prasad AB, Slotta DJ, Tolstoy I, Tyson GH, Zhao S, Hsu C-H, McDermott PF, Tadesse DA, Morales C, Simmons M, Tillman G, Wasilenko J, Folster JP, Klimke W. 2019. Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates. Antimicrob Agents Chemother 63:e00483-19. doi:10.1128/AAC.00483-19. PubMed DOI PMC

Carattoli A, Zankari E, García-Fernández A, Voldby Larsen M, Lund O, Villa L, Møller Aarestrup F, Hasman H. 2014. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother 58:3895–3903. doi:10.1128/AAC.02412-14. PubMed DOI PMC

Couvin D, Bernheim A, Toffano-Nioche C, Touchon M, Michalik J, Néron B, Rocha EPC, Vergnaud G, Gautheret D, Pourcel C. 2018. CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins. Nucleic Acids Res 46:W246–W251. doi:10.1093/nar/gky425. PubMed DOI PMC