Calcium carbonate particles: synthesis, temperature and time influence on the size, shape, phase, and their impact on cell hydroxyapatite formation
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34518864
DOI
10.1039/d1tb01072g
Knihovny.cz E-resources
- MeSH
- 3T3 Cells MeSH
- Hydroxyapatites chemistry metabolism MeSH
- Drug Delivery Systems MeSH
- Mice MeSH
- Osteoblasts drug effects metabolism MeSH
- Tissue Engineering MeSH
- Calcium Carbonate chemical synthesis chemistry MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hydroxyapatites MeSH
- Calcium Carbonate MeSH
To develop materials for drug delivery and tissue engineering and to study their efficiency with respect to ossification, it is necessary to apply physicochemical and biological analyses. The major challenge is labor-intensive data mining during synthesis and the reproducibility of the obtained data. In this work, we investigated the influence of time and temperature on the reaction yield, the reaction rate, and the size, shape, and phase of the obtained product in the completely controllable synthesis of calcium carbonate. We show that calcium carbonate particles can be synthesized in large quantities, i.e., in gram quantities, which is a substantial advantage over previously reported synthesis methods. We demonstrated that the presence of vaterite particles can dramatically stimulate hydroxyapatite (HA) production by providing the continued release of the main HA component - calcium ions - depending on the following particle parameters: size, shape, and phase. To understand the key parameters influencing the efficiency of HA production by cells, we created a predictive model by means of principal component analysis. We found that smaller particles in the vaterite state are best suited for HA growth (HA growth was 8 times greater than that in the control). We also found that the reported dependence of cell adhesion on colloidal particles can be extended to other types of particles that contain calcium ions.
Institute of Physical and Applied Chemistry Brno University of Technology Brno Czech Republic
NanoBioTechnology laboratory Faculty of Bioscience Engineering Ghent University 9000 Ghent Belgium
Science Medical Center Saratov State University Saratov 410012 Russian Federation
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