Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.

Masaryk University Faculty of Medicine Department of Histology and Embryology Brno Czech Republic; Reprofit International Clinic of Reproductive Medicine Brno Czech Republic

Masaryk University Faculty of Medicine Department of Histology and Embryology Brno Czech Republic; St Anne's University Hospital International Clinical Research Center Cell and Tissue Engineering Facility Brno Czech Republic

Masaryk University Faculty of Medicine Department of Histology and Embryology Brno Czech Republic; St Anne's University Hospital International Clinical Research Center Cell and Tissue Regeneration Brno Czech Republic

Reprofit International Clinic of Reproductive Medicine Brno Czech Republic

References provided by Crossref.org

The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy