INTRODUCTION: Cell therapy has the potential to improve symptoms and clinical outcomes in refractory angina (RFA). Further analyses are needed to evaluate factors influencing its therapeutic effectiveness. AIM: Assessment of electromechanical (EM) parameters of the left ventricle (LV) and investigation of correlation between EM parameters of the myocardium and response to CD133+ cell therapy. MATERIAL AND METHODS: Thirty patients with RFA (16 active and 14 placebo individuals) enrolled in the REGENT-VSEL trial underwent EM evaluation of the LV with intracardiac mapping system. The following parameters were analyzed: unipolar voltage (UV), bipolar voltage (BV), local linear shortening (LLS). Myocardial ischemia was evaluated with single-photon emission computed tomography (SPECT). The median value of each EM parameter was used for intra-group comparisons. RESULTS: Global EM parameters (UV, BV, LLS) of LV in active and placebo groups were 11.28 mV, 3.58 mV, 11.12%, respectively; 13.00 mV, 3.81 mV, 11.32%, respectively. EM characteristics analyzed at global and segmental levels did not predict response to CD133+ cell therapy in patients with RFA (Global UV, BV and LLS at rest R = -0.06; R = 0.2; R = -0.1 and at stress: R = 0.07, R = 0.09, R = -0.1, respectively; Segmental UV, BV, LLS at rest R = -0.2, R = 0.03, R = -0.4 and at stress R = 0.02, R = 0.2, R = -0.2, respectively). Multiple linear regression of the treated segments showed that only pre-injection SPECT levels were significantly correlated with post-injection SPECT, either at rest or stress (p < 0.05). CONCLUSIONS: Electromechanical characteristics of the left ventricle do not predict changes of myocardial perfusion by SPECT after cell therapy. Baseline SPECT results are only predictors of changes of myocardial ischemia observed at 4-month follow-up.

Department of Cardiac Surgery Medical University of Silesia Katowice Poland

Department of Cardiology and Structural Heart Disease Medical University of Silesia Katowice Poland

Department of Electrocardiology and Heart Failure Medical University of Silesia Katowice Poland

Department of Epidemiology Medical University of Silesia Katowice Poland

Department of General and Vascular Surgery Medical University of Silesia Katowice Poland

Department of Internal Medicine Cardioangiology St Anne's University Hospital Brno Czech Republic

Department of Nuclear Medicine John Paul 2 Hospital Jagiellonian University Medical College Krakow Poland

Department of Robotics and Mechatronics AGH University of Science and Technology Krakow Poland

IHU LIRYC Electrophysiology and Heart Modeling Institute Fondation Bordeaux Université Pessac France

Interventional Cardiac Electrophysiology Group International Clinical Research Center St Anne's University Hospital Brno Czech Republic

Univ Bordeaux INSERM UMR 1045 Cardiothoracic Research Center of Bordeaux Pessac France

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