Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s.

Amity Institute of Genome Engineering Amity University Uttar Pradesh Sector 125 Noida India

BIOSS Centre for Biological Signaling Studies University of Freiburg Freiburg Germany

Central European Institute of Technology Masaryk University Brno Czech Republic

Centre for Organismal Studies Heidelberg University of Heidelberg Heidelberg Germany

Department of Developmental Biology University of Hamburg Hamburg Germany

Institute Jean Pierre Bourgin INRAE AgroParisTech Université Paris Saclay Versailles France

Nara Institute of Science and Technology Nara Japan

RIKEN Center for Sustainable Resource Science Yokohama Japan

VIB UGent Center for Plant Systems Biology Gent Belgium

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A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis