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Almagro Armenteros JJ, Sønderby CK, Sønderby SK, Nielsen H, Winther O (2017) DeepLoc: prediction of protein subcellular localization using deep learning. Bioinformatics 33: 3387–3395
PubMed
Arama E, Agapite J, Steller H (2003) Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila . Dev Cell 4: 687–697
PubMed
Bagchi P, Inoue T, Tsai B (2016) EMC1‐dependent stabilization drives membrane penetration of a partially destabilized non‐enveloped virus. eLife 5: 1–23
PubMed
PMC
Bagchi P, Torres M, Qi L, Tsai B (2020) Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. Nat Commun 11: 1–15
PubMed
PMC
Bai L, You Q, Feng X, Kovach A, Li H (2020) Structure of the ER membrane complex, a transmembrane‐domain insertase. Nature 584: 475–478
PubMed
PMC
Barrows NJ, Anglero‐Rodriguez Y, Kim B, Jamison SF, Le Sommer C, McGee CE, Pearson JL, Dimopoulos G, Ascano M, Bradrick SS et al (2019) Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis. Sci Rep 9: 1–16
PubMed
PMC
Bateman A (2019) UniProt: A worldwide hub of protein knowledge. Nucleic Acids Res 47: D506–D515
PubMed
PMC
Borgese N, Colombo S, Pedrazzini E (2003) The tale of tail‐anchored proteins: coming from the cytosol and looking for a membrane. J Cell Biol 161: 1013–1019
PubMed
PMC
Brambillasca S, Yabal M, Soffientini P, Stefanovic S, Makarow M, Hegde RS, Borgese N (2005) Transmembrane topogenesis of a tail‐anchored protein is modulated by membrane lipid composition. EMBO J 24: 2533–2542
PubMed
PMC
Chen YN, Wu CH, Zheng Y, Li JJ, Wang JL, Wang YF (2015a) Knockdown of ATPsyn‐b caused larval growth defect and male infertility in Drosophila . Arch Insect Biochem Physiol 88: 144–154
PubMed
Chen Z, Chen HC, Montell C (2015b) TRP and rhodopsin transport depends on dual XPORT ER chaperones encoded by an operon. Cell Rep 13: 573–584
PubMed
PMC
Chitwood PJ, Juszkiewicz S, Guna A, Shao S, Hegde RS (2018) EMC is required to initiate accurate membrane protein topogenesis. Cell 175: 1–13
PubMed
PMC
Chitwood PJ, Hegde RS (2019) The role of EMC during membrane protein biogenesis. Trends Cell Biol 29: 1–14
PubMed
Chiu CF, Ghanekar Y, Frost L, Diao A, Morrison D, McKenzie E, Lowe M (2008) ZFPL1, a novel ring finger protein required for cis‐Golgi integrity and efficient ER‐to‐Golgi transport. EMBO J 27: 934–947
PubMed
PMC
Christianson JC, Olzmann JA, Shaler TA, Sowa ME, Bennett EJ, Richter CM, Tyler RE, Greenblatt EJ, Harper JW, Kopito RR (2011) Defining human ERAD networks through an integrative mapping strategy. Nat Cell Biol 14: 93–105
PubMed
PMC
Coelho JPL, Stahl M, Bloemeke N, Meighen‐Berger K, Alvira CP, Zhang Z‐R, Sieber SA, Feige MJ (2019) A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10: 1–10
PubMed
PMC
Costello JL, Castro IG, Camões F, Schrader TA, McNeall D, Yang J, Giannopoulou E‐A, Gomes S, Pogenberg V, Bonekamp NA et al (2017) Predicting the targeting of tail‐anchored proteins to subcellular compartments in mammalian cells. J Cell Sci 130: 1675–1687
PubMed
PMC
Coy‐Vergara J, Rivera‐Monroy J, Urlaub H, Lenz C, Schwappach B (2019) A trap mutant reveals the physiological client spectrum of TRC40. J Cell Sci 132: jcs230094
PubMed
PMC
Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2: 953–971
PubMed
Fagerberg L, Jonasson K, Von Heijne G, Uhlén M, Berglund L (2010) Prediction of the human membrane proteome. Proteomics 10: 1141–1149
PubMed
Guna A, Hegde RS (2018) Transmembrane domain recognition during membrane protein biogenesis and quality control. Curr Biol 28: R498–R511
PubMed
Guna A, Volkmar N, Christianson JC, Hegde RS (2018) The ER membrane protein complex is a transmembrane domain insertase. Science 359: 470–473
PubMed
PMC
Harel T, Yesil G, Bayram Y, Coban‐Akdemir Z, Charng W‐L, Karaca E, Al Asmari A, Eldomery M, Hunter J, Jhangiani S et al (2016) Monoallelic and biallelic variants in EMC1 identified in individuals with global developmental delay, hypotonia, scoliosis, and cerebellar atrophy. Am J Hum Genet 98: 562–570
PubMed
PMC
Hegde RS, Keenan RJ (2011) Tail‐anchored membrane protein insertion into the endoplasmic reticulum. Nat Rev Mol Cell Biol 12: 787–798
PubMed
PMC
Hiramatsu N, Tago T, Satoh T, Satoh AK (2019) ER membrane protein complex is required for the insertions of late‐synthesized transmembrane helices of Rh1 in Drosophila photoreceptors. Mol Biol Cell 30: 2890–2900
PubMed
PMC
Janer A, Prudent J, Paupe V, Fahiminiya S, Majewski J, Sgarioto N, Des RC, Forest A, Lin Z, Gingras A et al (2016) SLC 25 A 46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. EMBO Mol Med 8: 1–20
PubMed
PMC
Jonikas MC, Collins SR, Denic V, Oh E, Quan EM, Schmid V, Weibezahn J, Schwappach B, Walter P, Weissman JS et al (2009) Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum. Science 323: 1693–1697
PubMed
PMC
Käll L, Krogh A, Sonnhammer ELL (2005) An HMM posterior decoder for sequence feature prediction that includes homology information. Bioinformatics 21: 251–257
PubMed
Katanosaka K, Tokunaga F, Kawamura S, Ozaki K (1998) N‐Linked glycosylation of Drosophila rhodopsin occurs exclusively in the amino‐terminal domain and functions in rhodopsin maturation. FEBS Lett 424: 149–154
PubMed
Kriechbaumer V, Shaw R, Mukherjee J, Bowsher CG, Harrison AM, Abell BM (2009) Subcellular distribution of tail‐anchored proteins in arabidopsis. Traffic 10: 1753–1764
PubMed
Kutay U, Hartmann E, Rapoport TA (1993) A class of membrane proteins with a C‐terminal anchor. Trends Cell Biol 3: 72–75
PubMed
Lahiri S, Chao JT, Tavassoli S, Wong AKO, Choudhary V, Young BP, Loewen CJR, Prinz WA (2014) A conserved endoplasmic reticulum membrane protein complex (EMC) facilitates phospholipid transfer from the ER to mitochondria. PLoS Biol 12: e1001969
PubMed
PMC
Lakshminarayan R, Phillips BP, Binnian IL, Gomez‐Navarro N, Escudero‐Urquijo N, Warren AJ, Miller EA (2020) Pre‐emptive quality control of a misfolded membrane protein by ribosome‐driven effects. Curr Biol 30: 854–864
PubMed
PMC
Li Y, Zhao Y, Hu J, Xiao J, Qu L, Wang Z, Ma D, Chen Y (2013) A novel ER‐localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell Autophagy. Autophagy 9: 150–163
PubMed
PMC
Lin DL, Inoue T, Chen YJ, Chang A, Tsai B, Tai AW (2019) The ER membrane protein complex promotes biogenesis of dengue and Zika virus non‐structural multi‐pass transmembrane proteins to support infection. Cell Rep 27: 1666–1674
PubMed
PMC
Louie RJ, Guo J, Rodgers JW, White R, Shah NA, Pagant S, Kim P, Livstone M, Dolinski K, McKinney BA et al (2012) A yeast phenomic model for the gene interaction network modulating CFTR‐ΔF508 protein biogenesis. Genome Med 4: 103
PubMed
PMC
Ma Z, Liu Z, Huang X (2010) OSBP‐ and FAN‐mediated sterol requirement for spermatogenesis in Drosophila . Development 137: 3775–3784
PubMed
Mariappan M, Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJ (2011) The mechanism of membrane‐associated steps in tail‐anchored protein insertion. Nature 477: 61–69
PubMed
PMC
Mateja A, Paduch M, Chang HY, Szydlowska A, Kossiakoff AA, Hegde RS, Keenan RJ (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science 347: 1152–1155
PubMed
PMC
Miller‐Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM et al (2020) Structural and mechanistic basis of the EMC‐dependent biogenesis of distinct transmembrane clients. eLife 9: 1–47
PubMed
PMC
O’Donnell JP, Phillips BP, Yagita Y, Juszkiewicz S, Wagner A, Malinverni D, Keenan RJ, Miller EA, Hegde RS (2020) The architecture of EMC reveals a path for membrane protein insertion. eLife 9: 1–30
PubMed
PMC
O’Tousa JE (1992) Requirement of N‐linked glycosylation site in Drosophila rhodopsin. Vis Neurosci 8: 385–390
PubMed
Otsu N (1979) A threshold selection method from Gray‐level histograms. IEEE Trans Syst Man Cybern 9: 62–66
Pleiner T, Tomaleri GP, Januszyk K, Inglis AJ, Hazu M, Voorhees RM (2020) Structural basis for membrane insertion by the human ER membrane protein complex. Science 369: 433–436
PubMed
PMC
Rao M, Okreglak V, Chio US, Cho H, Walter P, Shan S‐O (2016) Multiple selection filters ensure accurate tail‐anchored membrane protein targeting. eLife 5: 1–24
PubMed
PMC
Richard M, Boulin T, Robert VJP, Richmond JE, Bessereau J‐L (2013) Biosynthesis of ionotropic acetylcholine receptors requires the evolutionarily conserved ER membrane complex. Proc Natl Acad Sci USA 110: E1055–E1063
PubMed
PMC
Rosenbaum EE, Brehm KS, Vasiljevic E, Liu CH, Hardie RC, Colley NJ (2011) XPORT‐dependent transport of TRP and rhodopsin. Neuron 72: 602–615
PubMed
PMC
Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ (2014) Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N‐glycan trimming in Drosophila melanogaster . PLoS Genet 10: e1004349
PubMed
PMC
Satoh T, Ohba A, Liu Z, Inagaki T, Satoh AK (2015) dPob/EMC is essential for biosynthesis of rhodopsin and other multi‐pass membrane proteins in Drosophila photoreceptors. eLife 4: 1–21
PubMed
PMC
Savidis G, McDougall WM, Meraner P, Perreira JM, Portmann JM, Trincucci G, John SP, Aker AM, Renzette N, Robbins DR et al (2016) Identification of Zika virus and dengue virus dependency factors using functional genomics. Cell Rep 16: 232–246
PubMed
Schindelin J, Arganda‐Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B et al (2012) Fiji: an open‐source platform for biological‐image analysis. Nat Methods 9: 676–682
PubMed
PMC
Schuldiner M, Metz J, Schmid V, Denic V, Rakwalska M, Schmitt HD, Schwappach B, Weissman JS (2008) The GET complex mediates insertion of tail‐anchored proteins into the ER membrane. Cell 134: 634–645
PubMed
PMC
Shen X, Kan S, Hu J, Li M, Lu G, Zhang M, Zhang S, Hou Y, Chen Y, Bai Y (2016) EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death Dis 7: e2043
PubMed
PMC
Shurtleff MJ, Itzhak DN, Hussmann JA, Schirle Oakdale NT, Costa EA, Jonikas M, Weibezahn J, Popova KD, Jan CH, Sinitcyn P et al (2018) The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. eLife 7: 1–23
PubMed
PMC
Stefanovic S, Hegde RS (2007) Identification of a targeting factor for posttranslational membrane protein insertion into the ER. Cell 128: 1147–1159
PubMed
Stefer S, Reitz S, Wang F, Wild K, Pang Y‐Y, Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F et al (2011) Structural basis for tail‐anchored membrane protein biogenesis by the Get3‐receptor complex. Science 333: 758–762
PubMed
PMC
Tang X, Snowball JM, Xu Y, Na C‐L, Weaver TE, Clair G, Kyle JE, Zink EM, Ansong C, Wei W et al (2017) EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest 127: 4314–4325
PubMed
PMC
Taylor MR, Kikkawa S, Diez‐Juan A, Ramamurthy V, Kawakami K, Carmeliet P, Brockerhoff SE (2005) The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells. Genetics 170: 263–273
PubMed
PMC
Tian S, Wu Q, Zhou B, Choi MY, Ding B, Yang W, Dong M (2019) Proteomic analysis identifies membrane proteins dependent on the ER membrane protein complex. Cell Rep 28: 2517–2526
PubMed
PMC
Tsirigos KD, Peters C, Shu N, Käll L, Elofsson A (2015) The TOPCONS web server for consensus prediction of membrane protein topology and signal peptides. Nucleic Acids Res 43: W401–W407
PubMed
PMC
Volkmar N, Thezenas ML, Louie SM, Juszkiewicz S, Nomura DK, Hegde RS, Kessler BM, Christianson JC (2019) The ER membrane protein complex promotes biogenesis of sterol‐related enzymes maintaining cholesterol homeostasis. J Cell Sci 132: jcs223453
PubMed
PMC
Volkmar N, Christianson JC (2020) Squaring the EMC – how promoting membrane protein biogenesis impacts cellular functions and organismal homeostasis. J Cell Sci 133: 1–14
PubMed
PMC
Wallin E, Von Heijne G (1998) Genome‐wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci 7: 1029–1038
PubMed
PMC
Wang F, Brown EC, Mak G, Zhuang J, Denic V (2010) A chaperone cascade sorts proteins for posttranslational membrane insertion into the endoplasmic reticulum. Mol Cell 40: 159–171
PubMed
PMC
Webel R, Menon I, Tousa JEO, Colley NJ (2000) Role of asparagine‐linked oligosaccharides in rhodopsin maturation and association with its molecular chaperone, NinaA. J Biol Chem 275: 24752–24759
PubMed
Wideman JG (2015) The ubiquitous and ancient ER membrane protein complex (EMC): tether or not? F1000Res 4: 624
PubMed
PMC
Xiong L, Zhang L, Yang Y, Li N, Lai W, Wang F, Zhu X, Wang T (2020) ER complex proteins are required for rhodopsin biosynthesis and photoreceptor survival in Drosophila and mice. Cell Death Differ 27: 646–661
PubMed
PMC
Zhao G, London E (2006) An amino acid “transmembrane tendency” scale that approaches the theoretical limit to accuracy for prediction of transmembrane helices: Relationship to biological hydrophobicity. Protein Sci 15: 1987–2001
PubMed
PMC
Zhou Y, Wu F, Zhang M, Xiong Z, Yin Q, Ru Y, Shi H, Li J, Mao S, Li Y et al (2018) EMC 10 governs male fertility via maintaining sperm ion balance. J Mol Cell Biol 6: 503–514
PubMed
PMC