The aim of the present study was to characterize bacteria of the genus Streptococcus isolated from the oral cavity of the guinea pig as well as to assess the significance of these microorganisms as potential veterinary and human pathogens. Sixty-two streptococcal isolates recovered from 27 clinically healthy guinea pigs were examined genotypically by sequencing the 16S rRNA and groEL genes. Among these isolates, only 13 could be assigned to a species described previously (mainly Streptococcus parasanguinis, S. mitis and S. suis), and the majority of the remaining ones differed considerably from the streptococcal species known to date (16S rRNA and groEL sequence similarities were < 97% and < 87%, respectively). Based on 16S rRNA sequences, these unidentified isolates were divided into seven groups (clades), of which clades I through III comprised most of the isolates examined and had also the widest distribution among guinea pig colonies. Upon groEL gene sequence analysis, however, members of the three clades grouped together without forming such distinct clusters. The remaining clades distinguished by 16S rRNA sequencing could also be discerned by the second gene, and they contained only a few isolates often restricted to one or a few animal colonies. The present work reveals that the guinea pig mouth is inhabited by a vast number of phylogenetically diverse, so far unrecognized populations of streptococci, most of them being apparently host-specific genomospecies. On the contrary, S. parasanguinis and S. mitis are also common human commensals and S. suis is a well-recognized zoonotic pathogen.

Department of Pathology Faculty of Veterinary Medicine Wroclaw University of Environmental and Life Sciences St Norwida 31 50 375 Wrocław Poland

Student of the Faculty of Veterinary Medicine University of Environmental and Life Sciences Wrocław Poland

Sub Department of Veterinary Microbiology Faculty of Veterinary Medicine University of Life Sciences in Lublin St Akademicka 12 20 033 Lublin Poland

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Abrahamian FM, Goldstein EJC. Microbiology of animal bite wound infections. Clin Microbiol Rev. 2011;24:231–246. doi: 10.1128/CMR.00041-10. PubMed DOI PMC

Coykendall AL. Proposal to elevate the subspecies of Streptococcus mutans to species status, based on their molecular composition. Int J Syst Bact. 1977;27:26–30. doi: 10.1099/00207713-27-1-26. DOI

Crowley EJ, King JM, Wilkinson T, Worgan HJ, Huson KM, Rose MT, McEwan NR. Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. PLoS ONE. 2017;12:e0165779. doi: 10.1371/journal.pone.0165779. PubMed DOI PMC

FEDIAF (2019) The European pet food industry: facts and figures. http://www.fediaf.org/ (accessed 10 November 2020)

Glazunova OO, Raoult D, Roux V. Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus. Int J Syst Evol Microbiol. 2009;59:2317–2322. doi: 10.1099/ijs.0.005488-0. PubMed DOI

Gottschalk M, Segura M, Xu J. Streptococcus suis infections in humans: the Chinese experience and the situation in North America. Animal Health Res Rev. 2007;8:29–l45. doi: 10.1017/S1466252307001247. PubMed DOI

Harmsen D, Dostal S, Roth A, Niemann S, Rothgänger J, Sammeth M, Albert J, Frosch M, Richter E. RIDOM: comprehensive and public sequence database for identification of Mycobacterium species. BMC Infect Dis. 2003;3:26. doi: 10.1186/1471-2334-3-26. PubMed DOI PMC

Kawamura Y, Hou XG, Sultana F, Miura H, Ezaki T. Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol. 1995;45:406–408. doi: 10.1099/00207713-45-2-406. PubMed DOI

Kim M, Oh H-S, Park S-C, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol. 2014;64:346–351. doi: 10.1099/ijs.0.059774-0. PubMed DOI

Król J, Wanecka A, Twardoń J, Mrowiec J, Dropińska A, Bania J, Podkowik M, Korzeniowska-Kowal A, Paściak M. Isolation of Staphylococcus microti from milk of dairy cows with mastitis. Vet Microbiol. 2016;182:163–169. doi: 10.1016/j.vetmic.2015.11.018. PubMed DOI

Kumar S, Tamura K, Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinf. 2004;5:150–163. doi: 10.1093/bib/5.2.150. PubMed DOI

Lion C, Conroy MC, Dupuy ML, Escande F. Pasteurella “SP” group infection after a guinea pig bite. Lancet. 1995;346:901–902. doi: 10.1016/S0140-6736(95)92741-7. PubMed DOI

Meredith A. Guinea pigs: common things are common. Vet Rec. 2015;177:198–199. doi: 10.1136/vr.h4465. PubMed DOI

Minarikova A, Hauptman K, Jeklova E, Knotek Z, Jekl V. Diseases in pet guinea pigs: a retrospective study in 1000 animals. Vet Rec. 2015;177:200. doi: 10.1136/vr.103053. PubMed DOI

Minarikova A, Hauptman K, Knotek Z, Jekl V. Microbial flora of odontogenic abscesses in pet guinea pigs. Vet Rec. 2016;179:331. doi: 10.1136/vr.103551. PubMed DOI

Naveen Kumar V, van der Linden M, Menon T, Nitsche-Schmitz DP. Viridans and bovis group streptococci that cause infective endocarditis in two regions with contrasting epidemiology. Int J Med Microbiol. 2014;304:262–268. doi: 10.1016/j.ijmm.2013.10.004. PubMed DOI

Palakawong Na Ayudthaya S, Hilderink LJ, van der Oost J, de Vos WM, Plugge CM. Streptococcus caviae sp. nov., isolated from guinea pig faecal samples. Int J Syst Evol Microbiol. 2017;67:1551–1556. doi: 10.1099/ijsem.0.001758. PubMed DOI

Saito M, Shinozaki-Kuwahara N, Hirasawa M, Takada K. Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants. Int J Syst Evol Microbiol. 2014;64:3288–3292. doi: 10.1099/ijs.0.063263-0. PubMed DOI

Shomer NH, Holcombe H, Harkness JE (2015) Biology and Diseases of Guinea Pigs. Laboratory Animal Medicine, 3rd edn. Epub 2015 Jul 10. PMCID: PMC7158311, pp 247–283. 10.1016/B978-0-12-409527-4.00006-7

Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today. 2006;33:152–155.

Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol. 1994;44:846–849. doi: 10.1099/00207713-44-4-846. DOI

Teng L-J, Hsueh P-R, Tsai J-C, Chen P-W, Hsu J-C, Lai H-C, Lee C-N, Ho S-W. groESL sequence determination, phylogenetic analysis, and species differentiation for viridians group streptococci. J Clin Microbiol. 2002;40:3172–3178. doi: 10.1128/JCM.40.9.3172-3178.2002. PubMed DOI PMC

Vela AI, Sánchez del Rey V, Zamora L, Casamayor A, Domínguez L, Fernández-Garayzábal JF. Streptococcus cuniculi sp. nov., isolated from the respiratory tract of wild rabbits. Int J Syst Evol Microbiol. 2014;64:2486–2490. doi: 10.1099/ijs.0.063123-0. PubMed DOI

Zhu H, Willcox MDP, Knox KW. A new species of oral Streptococcus isolated from Sprague-Dawley rats, Streptococcus orisratti sp. nov. Int J Syst Evol Microbiol. 2000;50:55–61. doi: 10.1099/00207713-50-1-55. PubMed DOI