Rhipicephalus camicasi Morel, Mouchet et Rodhain, 1976 is thought to be distributed across Africa, Arabian Peninsula and the Mediterranean region. It belongs to the Rhipicephalus sanguineus (Latreille, 1806) species complex. Mitochondrial genome sequences are becoming frequently used for the identification and differentiation of tick species. In the present study, the entire mitochondrial genome of R. cf. camicasi (~15 kb) collected from a camel in Saudi Arabia was sequenced and compared with mitogenomes of two species of Rhipicephalus Koch, 1844. The mitochondrial genome is 87.8% and 91.7% identical to the reference genome of R. sanguineus (sensu stricto, former "temperate lineage") and Rhipicephalus linnaei (Audouin, 1826) (former "tropical lineage"). The current study delivers a molecular reference for material that resembles R. camicasi. We propose to consider the current material, including the complete mitogenome, as the reference for R. camicasi, until a revision using topotypical material is available.

Faculty of Science and Humanities Shaqra University Ad Dawadimi Saudi Arabia

Sydney School of Veterinary Science Faculty of Science University of Sydney Sydney New South Wales Australia

Zobrazit více v PubMed

Alanazi A.D., Al-Mohammed H.I., Alyousif M.S., Said A.E., Salim B., Abdel-Shafy S., Shaapan R.M. 2019: Species diversity and seasonal distribution of hard ticks (Acari: Ixodidae) infesting mammalian hosts in various districts of Riyadh Province, Saudi Arabia. J. Med. Entomol. 56: 1027-1032. PubMed DOI

el-Azazy O.M., Scrimgeour E.M. 1997: Crimean-Congo haemorrhagic fever virus infection in the western province of Saudi Arabia. Trans. R. Soc. Trop. Med. Hyg. 91: 275-278. PubMed DOI

Bernt M., Donath A., Juhling F., Externbrink F., Florentz C., Fritzsch G., Putz J., Middendorf M., Stadler P.F. 2013: MITOS: improved de novo metazoan mitochondrial genome annotation. Mol. Phylogenet. Evol. 69: 313-319. PubMed DOI

Camicas J.-L., Hervy J.-P., Adam F., Morel P.C. (Eds.) 1998: Les Tiques du Monde (Acarida, Ixodida). Nomenclature, Stades Décrits, Hôtes, Répartition. Orstom, Paris, 233 pp.

Chandra S., Smith K., Alanazi A.D., Alyousif M.S., Emery D., Slapeta J. 2019: Rhipicephalus sanguineus sensu lato from dogs and dromedary camels in Riyadh, Saudi Arabia: low prevalence of vector-borne pathogens in dogs detected using multiplexed tandem PCR panel. Folia Parasitol. 66: 007. PubMed DOI

Estrada-Peña A. 2017: Rhipicephalus camicasi Morel, Mouchet and Rodhain, 1976. In: A. Estrada-Peña, A.D. Mihalca, T. Petney (Eds.), Ticks of Europe and North Africa: A Guide to Species Identification. Springer International Publishing, Cham, Switzerland, pp. 317-319. DOI

Estrada-Peña A., Bouattour A., Camicas J.-L., Walker A.R. (Eds.) 2004: Ticks of Domestic Animals in the Mediterranean Region: A Guide to Identification of Species. University of Zaragoza, Zaragoza, Spain, 131 pp.

Gray J., Dantas-Torres F., Estrada-Pena A., Levin M. 2013: Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 4: 171-180. PubMed DOI

Hahn C., Bachmann L., Chevreux B. 2013: Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads - a baiting and iterative mapping approach. Nucl. Acids Res. 41: e129. PubMed DOI

Harrison A., Robb G.N., Alagaili A.N., Hastriter M.W., Apanaskevich D.A., Ueckermann E.A., Bennett N.C. 2015: Ectoparasite fauna of rodents collected from two wildlife research centres in Saudi Arabia with discussion on the implications for disease transmission. Acta Trop. 147: 1-5. PubMed DOI

Jia N., Wang J., Shi W., Du L., Sun Y., Zhan W., Jiang J.F., Wang Q., Zhang B., Ji P., Bell-Sakyi L., Cui X.M., Yuan T.T., Jiang B.G., Yang W.F., Lam T.T., Chang Q.C., Ding S.J., Wang X.J., Zhu J.G., Ruan X.D., Zhao L., Wei J.T., Ye R.Z., Que T.C., Du C.H., Zhou Y.H., Cheng J.X., Dai P.F., Guo W.B., Han X.H., Huang E.J., Li L.F., Wei W., Gao Y.C., Liu J.Z., Shao H.Z., Wang X., Wang C.C., Yang T.C., Huo Q.B., Li W., Chen H.Y., Chen S.E., Zhou L.G., Ni X.B., Tian J.H., Sheng Y., Liu T., Pan Y.S., Xia L.Y., Li J., Tick G., Microbiome C., Zhao F., Cao W.C. 2020: Large-scale comparative analyses of tick genomes elucidate their genetic diversity and vector capacities. Cell 182: 1328-1340 e1313. PubMed DOI

Kumar S., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549. PubMed DOI

Mans B.J., Featherston J., Kvas M., Pillay K.A., de Klerk D.G., Pienaar R., de Castro M.H., Schwan T.G., Lopez J.E., Teel P., Perez de Leon A.A., Sonenshine D.E., Egekwu N.I., Bakkes D.K., Heyne H., Kanduma E.G., Nyangiwe N., Bouattour A., Latif A.A. 2019: Argasid and ixodid systematics: implications for soft tick evolution and systematics, with a new argasid species list. Ticks Tick Borne Dis. 10: 219-240. PubMed DOI

Morel P.C., Mouchet J., Rodhain F. 1976: Description of Rhipicephalus camicasi n. sp. (Aearidae, Ixodida) of subdesert steppes of the plain of Afar. Rev. Elev. Med. Vet. Pays Trop. 29: 337-340. PubMed DOI

Ravi A., Ereqat S., Al-Jawabreh A., Abdeen Z., Abu Shamma O., Hall H., Pallen M.J., Nasereddin A. 2019: Metagenomic profiling of ticks: Identification of novel rickettsial genomes and detection of tick-borne canine parvovirus. PloS Negl. Trop. Dis. 13: e0006805. PubMed DOI

Šlapeta J., Chandra S., Halliday B. 2021: The "tropical lineage" of the brown dog tick Rhipicephalus sanguineus sensu lato identified as Rhipicephalus linnaei (Audouin, 1826). Int. J. Parasitol. 51: 431-436. PubMed DOI

Walker J., Keirans J., Horak I. (Eds.) 2000: The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World. Cambridge University Press, Cambridge, United Kingdom, 221 pp. DOI

Wang T., Zhang S., Pei T., Yu Z., Liu J. 2019: Tick mitochondrial genomes: structural characteristics and phylogenetic implications. Parasit. Vectors 12: 451. PubMed DOI

The "southeastern Europe" lineage of the brown dog tick Rhipicephalus sanguineus (sensu lato) identified as Rhipicephalus rutilus Koch, 1844: Comparison with holotype and generation of mitogenome reference from Israel